Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 845799..846453 Replicon chromosome
Accession NZ_CP049112
Organism Escherichia coli strain WF5-5-T2

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag G6R06_RS04145 Protein ID WP_000244777.1
Coordinates 846046..846453 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G6R06_RS04140 Protein ID WP_000354046.1
Coordinates 845799..846065 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6R06_RS04115 (840968) 840968..841711 + 744 WP_000951964.1 SDR family oxidoreductase -
G6R06_RS04120 (841768) 841768..843201 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
G6R06_RS04125 (843246) 843246..843557 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
G6R06_RS04130 (843721) 843721..844380 + 660 WP_000250274.1 hemolysin III family protein -
G6R06_RS04135 (844576) 844576..845556 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
G6R06_RS04140 (845799) 845799..846065 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G6R06_RS04145 (846046) 846046..846453 + 408 WP_000244777.1 protein YgfX Toxin
G6R06_RS04150 (846493) 846493..847014 - 522 WP_001055874.1 flavodoxin FldB -
G6R06_RS04155 (847126) 847126..848022 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G6R06_RS04160 (848047) 848047..848757 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G6R06_RS04165 (848763) 848763..850496 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T150554 WP_000244777.1 NZ_CP049112:846046-846453 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T150554 NZ_CP064785:c137222-137064 [Klebsiella sp. BDA134-6]
ATGAAACTGCCGCGAAGTACCCTGCTATGGTGTGTATTAATAGTGTGTCTTACATTGCTGATATTCACTTACCTGACCCG
AAAATCGCTCTGCGAAATTCGGTACAGGGACACGAACAGGGAGGTGGCGGCTTTTATGGCCTACGAATCCGCTAAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT150554 WP_000354046.1 NZ_CP049112:845799-846065 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT150554 NZ_CP064785:c137399-137284 [Klebsiella sp. BDA134-6]
GGCGCTTGAGGCTTTTTGCCTCATGACGTGAAGGTGGTTTGTTGCCAGGTTGTGTGGCAGAAAGAAGAAAGCCCCGTAGG
AAGTTAATTTTCATTAACCAACCACACGAGGCCATC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References