Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 198328..199951 Replicon plasmid pMCR_NDM
Accession NZ_CP049111
Organism Escherichia coli strain WF5-5-T1

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag G5631_RS23530 Protein ID WP_000088046.1
Coordinates 198328..199653 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag G5631_RS23535 Protein ID WP_024136327.1
Coordinates 199658..199951 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5631_RS23500 (G5631_23325) 194312..195169 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
G5631_RS23505 (G5631_23330) 195185..195493 - 309 WP_001224687.1 hypothetical protein -
G5631_RS23510 (G5631_23335) 196042..196467 - 426 WP_001043843.1 hypothetical protein -
G5631_RS23515 (G5631_23340) 196721..197536 - 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
G5631_RS23520 (G5631_23345) 197549..197959 - 411 WP_000985911.1 hypothetical protein -
G5631_RS23525 (G5631_23350) 198061..198267 - 207 WP_000134171.1 hypothetical protein -
G5631_RS23530 (G5631_23355) 198328..199653 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
G5631_RS23535 (G5631_23360) 199658..199951 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
G5631_RS23540 (G5631_23365) 200555..200947 - 393 WP_000341066.1 hypothetical protein -
G5631_RS23545 (G5631_23370) 201320..201661 + 342 WP_001151575.1 hypothetical protein -
G5631_RS23550 (G5631_23375) 201753..201944 + 192 WP_000398480.1 hypothetical protein -
G5631_RS23555 (G5631_23380) 201994..202275 + 282 WP_000077926.1 hypothetical protein -
G5631_RS23560 202616..202747 - 132 WP_000374059.1 hypothetical protein -
G5631_RS23565 (G5631_23385) 202838..204079 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-4 / sul1 / qacE / aadA2 / dfrA12 / aac(3)-IId / blaTEM-1B / fosA3 / blaCTX-M-14 / mcr-1.1 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / sul2 / aph(4)-Ia / aac(3)-IVa / mph(A) / tet(M) - 1..436531 436531


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T150548 WP_000088046.1 NZ_CP049111:c199653-198328 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T150548 NZ_CP064784:1158443-1158700 [Klebsiella sp. BDA134-6]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCACGTTCGCGATACATC
AGGCCGCGTCACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCCTACCGCATCAATACCACCGGGCTGAACAACGTCCGCGCA
CAGCTGGACAATCGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT150548 WP_024136327.1 NZ_CP049111:c199951-199658 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT150548 NZ_CP064784:1158711-1159262 [Klebsiella sp. BDA134-6]
ATGACAAACCACAGCTTCACTTTTCATGTCGCCTCTGAAGGCGATACCGATGACATTCTCAATGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCTCTTTGCTCAACGATGAGAGCGCCTGCCCGACGTTATCGCTGCTGGCCA
GGCATAACGGAAAGGCGGTTGGGCATATTTTATTCACCCGGGCGATGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTGGCGGTGGTGCCGGAATACCAGGGCGCGGGCGTTGGCGGAGCGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCCATAGGAAGCCAGACGGTATTTGTTCTGGGCCACGCCACTTACTACCCCAGGCACGGGTTTGAGCCATGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCGATTCCCGAGGCGCATAAAGCCTGCTGGATGCTACAGCGGCTTTCGTCT
CAGCCGCTGGGGCGAACGGGCCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References