Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4105792..4106289 Replicon chromosome
Accession NZ_CP049110
Organism Escherichia coli strain WF5-5-T1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G5631_RS20130 Protein ID WP_001173343.1
Coordinates 4105792..4105965 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag G5631_RS20135 Protein ID WP_000398619.1
Coordinates 4105993..4106289 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5631_RS20120 (4102462) 4102462..4103919 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
G5631_RS20125 (4104156) 4104156..4105673 + 1518 WP_001295074.1 lysine--tRNA ligase -
G5631_RS20130 (4105792) 4105792..4105965 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G5631_RS20135 (4105993) 4105993..4106289 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G5631_RS20140 (4106516) 4106516..4106788 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
G5631_RS20145 (4106800) 4106800..4107030 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
G5631_RS20150 (4107211) 4107211..4108842 + 1632 WP_001216477.1 sensor histidine kinase -
G5631_RS20155 (4108839) 4108839..4109558 + 720 Protein_3935 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T150546 WP_001173343.1 NZ_CP049110:c4105965-4105792 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T150546 NZ_CP064781:2176372-2176503 [Azospira restricta]
ATGATCGCGCTCGCGCCAAAGACCGGGATTCTGTACTACGTGGCGTTTGTGGATCGTGGCAAAGTGCGAAGGATCATCAG
CCTTCGCCGCGCCAATCGACGTGAGGTGAAACACTATGTCGAGAACTTCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT150546 WP_000398619.1 NZ_CP049110:c4106289-4105993 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT150546 NZ_CP064781:2176520-2176786 [Azospira restricta]
ATGCCTACCGTGGAAGAGGACAGGGCCATTACCGCAGCGGCCAGGAGTGATCCTGATGCACAGCCGCTCACGCCCAAGCA
ACTGAAGACCATGGTTCCGCTGAAGGCCCTGCGTGGCCGGCCGAAATCCGAGAACAAGAAGCTGCTCGTATCCGTGCGCT
ACAGCCCCGAGGTCATTGCGTACTTCAAGTCCACCGGAGAGGGTTGGCAATCGCTCATGGACAGCGTGCTGCGCAAGTAC
GTGGCGCGCCATTCGCGTAGCGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References