Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 3497405..3498084 | Replicon | chromosome |
| Accession | NZ_CP049110 | ||
| Organism | Escherichia coli strain WF5-5-T1 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | P77692 |
| Locus tag | G5631_RS17095 | Protein ID | WP_000854672.1 |
| Coordinates | 3497405..3497746 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | Q47684 |
| Locus tag | G5631_RS17100 | Protein ID | WP_000070395.1 |
| Coordinates | 3497767..3498084 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G5631_RS17070 (3492991) | 3492991..3493083 | + | 93 | Protein_3334 | sigma factor-binding protein Crl | - |
| G5631_RS17075 (3493122) | 3493122..3494177 | - | 1056 | WP_103119219.1 | phosphoporin PhoE | - |
| G5631_RS17080 (3494465) | 3494465..3495568 | + | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| G5631_RS17085 (3495580) | 3495580..3496833 | + | 1254 | WP_000893278.1 | glutamate-5-semialdehyde dehydrogenase | - |
| G5631_RS17095 (3497405) | 3497405..3497746 | - | 342 | WP_000854672.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| G5631_RS17100 (3497767) | 3497767..3498084 | - | 318 | WP_000070395.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| G5631_RS17105 (3498103) | 3498103..3498324 | - | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| G5631_RS17110 (3498333) | 3498333..3498809 | - | 477 | WP_000811693.1 | RadC family protein | - |
| G5631_RS17115 (3498825) | 3498825..3499283 | - | 459 | WP_000211838.1 | antirestriction protein | - |
| G5631_RS17120 (3499381) | 3499381..3499620 | - | 240 | WP_000194654.1 | DUF905 family protein | - |
| G5631_RS17125 (3499697) | 3499697..3500164 | - | 468 | WP_001547765.1 | protein YkfB | - |
| G5631_RS17130 (3500187) | 3500187..3500630 | - | 444 | WP_000824223.1 | lipoprotein YafY | - |
| G5631_RS17135 (3500630) | 3500630..3500857 | - | 228 | WP_001548158.1 | protein YpjK | - |
| G5631_RS17140 (3500853) | 3500853..3501044 | - | 192 | Protein_3347 | DeoR family transcriptional regulator | - |
| G5631_RS17145 (3501261) | 3501261..3502082 | - | 822 | WP_000197389.1 | DUF932 domain-containing protein | - |
| G5631_RS17150 (3502174) | 3502174..3503037 | - | 864 | WP_001065553.1 | GTPase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC | 3492285..3569614 | 77329 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12905.01 Da Isoelectric Point: 9.6543
>T150539 WP_000854672.1 NZ_CP049110:c3497746-3497405 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T150539 NZ_CP064780:449757-449891 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11939.59 Da Isoelectric Point: 6.7391
>AT150539 WP_000070395.1 NZ_CP049110:c3498084-3497767 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT150539 NZ_CP064780:449541-449693 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|