Detailed information of TA system TA150539

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3497405..3498084
• Replicon: chromosome
• Accession: NZ_CP049110
• Organism: Escherichia coli strain WF5-5-T1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: G5631_RS17095
• Protein ID: WP_000854672.1
• Coordinates: 3497405..3497746 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: G5631_RS17100
• Protein ID: WP_000070395.1
• Coordinates: 3497767..3498084 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5631_RS17070 (3492991)	3492991..3493083	+	93	Protein_3334	sigma factor-binding protein Crl	-
G5631_RS17075 (3493122)	3493122..3494177	-	1056	WP_103119219.1	phosphoporin PhoE	-
G5631_RS17080 (3494465)	3494465..3495568	+	1104	WP_001285288.1	glutamate 5-kinase	-
G5631_RS17085 (3495580)	3495580..3496833	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
G5631_RS17095 (3497405)	3497405..3497746	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
G5631_RS17100 (3497767)	3497767..3498084	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
G5631_RS17105 (3498103)	3498103..3498324	-	222	WP_000691994.1	DUF987 domain-containing protein	-
G5631_RS17110 (3498333)	3498333..3498809	-	477	WP_000811693.1	RadC family protein	-
G5631_RS17115 (3498825)	3498825..3499283	-	459	WP_000211838.1	antirestriction protein	-
G5631_RS17120 (3499381)	3499381..3499620	-	240	WP_000194654.1	DUF905 family protein	-
G5631_RS17125 (3499697)	3499697..3500164	-	468	WP_001547765.1	protein YkfB	-
G5631_RS17130 (3500187)	3500187..3500630	-	444	WP_000824223.1	lipoprotein YafY	-
G5631_RS17135 (3500630)	3500630..3500857	-	228	WP_001548158.1	protein YpjK	-
G5631_RS17140 (3500853)	3500853..3501044	-	192	Protein_3347	DeoR family transcriptional regulator	-
G5631_RS17145 (3501261)	3501261..3502082	-	822	WP_000197389.1	DUF932 domain-containing protein	-
G5631_RS17150 (3502174)	3502174..3503037	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	3492285..3569614	77329

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T150539 WP_000854672.1 NZ_CP049110:c3497746-3497405 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T150539 NZ_CP064780:449757-449891 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT150539 WP_000070395.1 NZ_CP049110:c3498084-3497767 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT150539 NZ_CP064780:449541-449693 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684