150523

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 1107856..1108523 Replicon chromosome
Accession NZ_CP049110
Organism Escherichia coli strain WF5-5-T1

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag G5631_RS05360 Protein ID WP_001094400.1
Coordinates 1107856..1108185 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag G5631_RS05365 Protein ID WP_000072690.1
Coordinates 1108206..1108523 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5631_RS05355 (1102912) 1102912..1107492 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
G5631_RS05360 (1107856) 1107856..1108185 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
G5631_RS05365 (1108206) 1108206..1108523 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
G5631_RS05370 (1108561) 1108561..1108761 - 201 WP_001395452.1 DUF987 domain-containing protein -
G5631_RS05375 (1108770) 1108770..1109252 - 483 WP_001407480.1 RadC family protein -
G5631_RS05380 (1109261) 1109261..1109719 - 459 WP_000211841.1 antirestriction protein -
G5631_RS05385 (1109732) 1109732..1109854 + 123 WP_001407482.1 hypothetical protein -
G5631_RS05390 (1109822) 1109822..1110006 - 185 Protein_1051 DUF905 family protein -
G5631_RS05395 (1110617) 1110617..1112320 - 1704 WP_000896263.1 protein YfjW -
G5631_RS05400 (1112462) 1112462..1113471 + 1010 Protein_1053 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - luxS 1054860..1129479 74619


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T150523 WP_001094400.1 NZ_CP049110:c1108185-1107856 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T150523 NZ_CP064768:c4725406-4724873 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT150523 WP_000072690.1 NZ_CP049110:c1108523-1108206 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT150523 NZ_CP064768:c4725683-4725417 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
AlphaFold DB P52141

References