Detailed information of TA system TA150515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TacT2-ataR/DUF1778(antitoxin)
• Location: 1890356..1891155
• Replicon: chromosome
• Accession: NZ_CP049109
• Organism: Sphingosinithalassobacter sp. zrk23

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: G5C33_RS09290
• Protein ID: WP_165328796.1
• Coordinates: 1890661..1891155 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: G5C33_RS09285
• Protein ID: WP_165326950.1
• Coordinates: 1890356..1890643 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5C33_RS09240	1885557..1886246	-	690	WP_165326945.1	DNA methylase	-
G5C33_RS09245	1886243..1886458	-	216	WP_165326450.1	hypothetical protein	-
G5C33_RS09250	1886890..1887309	+	420	WP_165326451.1	MucR family transcriptional regulator	-
G5C33_RS09260	1887459..1888043	-	585	WP_165326452.1	tyrosine-type recombinase/integrase	-
G5C33_RS09265	1888260..1888493	+	234	WP_165326453.1	hypothetical protein	-
G5C33_RS09270	1888535..1888921	+	387	WP_165326947.1	hypothetical protein	-
G5C33_RS09275	1888918..1889820	-	903	WP_165326948.1	hypothetical protein	-
G5C33_RS09280	1890023..1890298	+	276	WP_165326949.1	hypothetical protein	-
G5C33_RS09285	1890356..1890643	+	288	WP_165326950.1	DUF1778 domain-containing protein	Antitoxin
G5C33_RS09290	1890661..1891155	+	495	WP_165328796.1	GNAT family N-acetyltransferase	Toxin
G5C33_RS09295	1891218..1891520	+	303	WP_206518668.1	hypothetical protein	-
G5C33_RS09300	1891572..1892999	-	1428	WP_165326952.1	relaxase	-
G5C33_RS09305	1892996..1893370	-	375	WP_165328797.1	plasmid mobilization relaxosome protein MobC	-
G5C33_RS09315	1893781..1894860	-	1080	WP_165326953.1	tyrosine-type recombinase/integrase	-
G5C33_RS09320	1894863..1895060	-	198	WP_165326954.1	hypothetical protein	-
G5C33_RS09325	1895057..1895356	-	300	WP_165326955.1	hypothetical protein	-
G5C33_RS09330	1895461..1895904	-	444	WP_165326956.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1870865..1892999	22134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17491.33 Da
• Isoelectric Point: 10.1403

>T150515 WP_165328796.1 NZ_CP049109:1890661-1891155 [Sphingosinithalassobacter sp. zrk23]
ISAPVLLNGTHRLEEFTCGIANLDDWLKRRALANQTGGASRTYVVAQDSRVIAYYCLASGGIAAIEAPGRIRRNMPDPIP
IAVLGRLAVDSGWQGKGLGVALLQDAVLRTAQAAEIVGIRALVVHALSDAAKAFYLRFGFIESPSNPMTLLLSLKGFANP
PKPR

Nucleotide

• Length: 495 bp

>T150515 NZ_CP064767:c3312855-3312505 [Weizmannia coagulans]
TTGATTGTTAAGCGTGGAGACGTGTACTTCGCCGATCTATCTCCGGTGGTCGGCTCCGAGCAGGGAGGCGTACGTCCTGT
CCTCATTTTACAAAACGACATTGGAAACCGTTTTAGCCCCACTGTCATTGTTGCCGCGATAACAGCGCAAATCCAGAAAG
CAAAATTGCCCACGCATGTGGAAATTGATGCAAAGCGTTACGGTTTGGAAAGGGATTCTGTGATATTGCTTGAACAAATA
CGCACAATCGATAAACAACGCTTAACGGATAAGATTACGCATCTTGATGAAGAAGTCATGGAAAAAATTGACGACGCCCT
GCAAATCAGCCTGGGACTGGTCGAATTTTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10763.10 Da
• Isoelectric Point: 5.8275

>AT150515 WP_165326950.1 NZ_CP049109:1890356-1890643 [Sphingosinithalassobacter sp. zrk23]
MVQTTPNTENHAKPVNLRVRPDVRALIDQAARLQGKSRSDFMIDAARRAAEDALLNQTLIRVDQDDYDRFLALLDQPASG
EGFARLMNATRPWEQ

Nucleotide

• Length: 288 bp

>AT150515 NZ_CP064767:c3313138-3312860 [Weizmannia coagulans]
GTGTCTGAACCTATTTCAACAGAAATTTTAGTCCGCTTGCCGCAGCAGCTGTTGACGGAATTGGACGGATTTGCTGAAAC
TGAAAATATGAATCGCAATGAATTAATTTACCGTGCCACAAAAATGTATTTAAAAGAACGGAAAAAACGCCAAATCCGCG
AAACGATGAGGCGTGGTTACATGGAAATGGCAAAAATTAATTTGGCGATTGCCTCCGAAGCGATCCAGGCTGAATACGAA
GCAGAACATACAGTTGAAAGCCTAGTAAGCGGGGGGTAA