Detailed information of TA system TA150511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 7959..8452
• Replicon: plasmid pCTX-M-15
• Accession: NZ_CP049103
• Organism: Escherichia coli strain EC28

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: G5V60_RS25240
• Protein ID: WP_001230709.1
• Coordinates: 7959..8213 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: G5V60_RS25245
• Protein ID: WP_000151456.1
• Coordinates: 8210..8452 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5V60_RS25205	3534..3695	+	162	WP_001434039.1	type I toxin-antitoxin system Hok family toxin	-
G5V60_RS25210	3844..4440	+	597	WP_000125165.1	hypothetical protein	-
G5V60_RS25780	4458..4742	-	285	WP_001162371.1	hypothetical protein	-
G5V60_RS25220	4816..5481	-	666	WP_015387337.1	P-loop NTPase	-
G5V60_RS25225	6068..6397	+	330	WP_000855119.1	hypothetical protein	-
G5V60_RS25230	6801..7316	+	516	WP_001025393.1	J domain-containing protein	-
G5V60_RS25235	7420..7962	+	543	WP_001083904.1	hypothetical protein	-
G5V60_RS25240	7959..8213	+	255	WP_001230709.1	hypothetical protein	Toxin
G5V60_RS25245	8210..8452	+	243	WP_000151456.1	hypothetical protein	Antitoxin
G5V60_RS25250	8589..8921	+	333	WP_001393253.1	WbuC family cupin fold metalloprotein	-
G5V60_RS25255	8968..9843	-	876	WP_000239590.1	class A extended-spectrum beta-lactamase CTX-M-15	-
G5V60_RS25260	10096..11358	-	1263	WP_000608644.1	IS1380-like element ISEc9 family transposase	-
G5V60_RS25265	11743..11992	+	250	Protein_14	hypothetical protein	-
G5V60_RS25270	12108..12395	+	288	WP_000673430.1	hypothetical protein	-
G5V60_RS25275	12405..12821	+	417	WP_053882499.1	phage tail protein	-
G5V60_RS25280	12818..13129	+	312	WP_001310436.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-15	-	1..63295	63295
	-	flank	IS/Tn	-	-	2389..3462	1073
	-	flank	IS/Tn	blaCTX-M-15	-	8968..11358	2390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-133)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T150511 WP_001230709.1 NZ_CP049103:7959-8213 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T150511 NZ_CP064760:c2503664-2503236 [Microbacterium schleiferi]
GTGAAACTCGTTGATGCCAATGTCCTGCTCTACGCCGTCGACGCGGATGCGCGTCATCACACAGAAGCTAAGGCCTGGCT
CGACACCGCTCTGAATTCGAGGGAGACTGTCGCGATCCCGTGGGTATGCGCGTTGGCTTTTGTCCGCCTGACAACGAGTT
CTCGGATCTACCCGAATCCGCTGCCGGTCGACCGTGCTTTCGACATCCTCAACGGATGGATGAGCCGATCTAGTGTCATC
AGCCTTGAGCCAGGAAGCGGACACGCGGACAGGATGCGCACGCTTCTTGCCGAGGTGGGAACCGGCGGAAATCTCGTCAC
GGATGCCCACCTCGCCGCGCTCGCGGTCGAGAATCGCGCAACGATCGTGACGTTCGACGGCGATTTTGCGCGATTCCCGG
GCGTTGTGTGGCACCGACCCGGGGACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT150511 WP_000151456.1 NZ_CP049103:8210-8452 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT150511 NZ_CP064760:c2503912-2503661 [Microbacterium schleiferi]
GTGCGAACCACGATCACGCTGACATCGGCTGCAGAGGAGGCCGTGCGCAAGGTCATGCGTGAACGCGGCCTATCGTTCAA
AGACGCCGTGAACTCGGCGATCCTCGCTGGTCTGGATCCCGGCCCGGCATCCGCGTACGAAACGCCGGTTCACAGGCTCG
GAAGGGCGCGCATGCCGCTGGACCGCGCTCTCGCTGTTGCAGCCGACCTCGAAGACGAAGCCATCACTCTGAAGCAAGAG
CTCGCGAAGTGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5