Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 145697..146340 Replicon plasmid p2
Accession NZ_CP049102
Organism Escherichia coli strain EC28

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag G5V60_RS25100 Protein ID WP_001034044.1
Coordinates 145924..146340 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag G5V60_RS25095 Protein ID WP_001261286.1
Coordinates 145697..145927 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5V60_RS25080 140834..141064 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
G5V60_RS25085 141061..141477 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
G5V60_RS25090 141522..145316 - 3795 WP_001144731.1 hypothetical protein -
G5V60_RS25095 145697..145927 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G5V60_RS25100 145924..146340 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
G5V60_RS25105 146415..147980 + 1566 WP_001128474.1 AAA family ATPase -
G5V60_RS25110 147965..148987 + 1023 WP_000361402.1 helicase UvrD -
G5V60_RS25115 149241..149938 - 698 Protein_153 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..168051 168051
- flank IS/Tn - - 149241..149744 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T150510 WP_001034044.1 NZ_CP049102:145924-146340 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T150510 NZ_CP064760:c1423802-1423455 [Microbacterium schleiferi]
TTGCGACGCGGTCACGTCGTGCTGGTCGATCTCGATCCAGCCGTCGGATCCGAAGCCGCGCGAACTCGCCCGGCCATCGT
TGTGAGCAACGCGACGGCAAACCTCAGCGCCGCGCGCACCGGCCACGGGGTGATCACGGTCGTGCCAGTGACCTCGAATA
TCTCGACCGTGCATCCGTTTCAGGTGCTTCTCTCGCCGGAACAGTCGGGACTCCCCCAGCCCAGCAAAGCCCAGGCAGAA
CAGGTGCGCGCAGTGTCGATTTCGCGCGTGAGCCGCGTCACCGGACGCATCACGGCTGAATCGCTCGGCGCTCTCGATGA
CGCCCTGCGGCTTCACCTGTCGTTGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT150510 WP_001261286.1 NZ_CP049102:145697-145927 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT150510 NZ_CP064760:c1424032-1423820 [Microbacterium schleiferi]
GTGAAAGTCAGCCTCAGCCTCAGCACCGATGACCTCGCGTTCCTCGACGCCCAGACTCGAACTGGCGTCTACTCCAGTCG
GTCCGCTGCGGTGCAAGACGCCGTGCGGGCGCTGCGCGAAGAACGACTCGCGGATGCCTACGCCGATGCCTTCGCCGAAC
CTGGGGACGACGCGTGGGATGCGGCATCCGGCGACGGACTTGCGCGGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References