Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 140834..141477 Replicon plasmid p2
Accession NZ_CP049102
Organism Escherichia coli strain EC28

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag G5V60_RS25085 Protein ID WP_001034046.1
Coordinates 141061..141477 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag G5V60_RS25080 Protein ID WP_001261278.1
Coordinates 140834..141064 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5V60_RS25050 136344..136649 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
G5V60_RS25055 136651..136869 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
G5V60_RS25060 137437..137949 + 513 WP_000151784.1 hypothetical protein -
G5V60_RS25065 137983..139116 - 1134 WP_000545984.1 DUF3800 domain-containing protein -
G5V60_RS25070 139283..140056 - 774 WP_000905949.1 hypothetical protein -
G5V60_RS25075 140069..140569 - 501 WP_000528932.1 hypothetical protein -
G5V60_RS25080 140834..141064 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G5V60_RS25085 141061..141477 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
G5V60_RS25090 141522..145316 - 3795 WP_001144731.1 hypothetical protein -
G5V60_RS25095 145697..145927 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
G5V60_RS25100 145924..146340 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..168051 168051


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T150509 WP_001034046.1 NZ_CP049102:141061-141477 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T150509 NZ_CP064760:c1252702-1252451 [Microbacterium schleiferi]
ATGGCCGCCATCACCGCGACCGAAGCACGAAAGAGTCTGTTCGGGCTCATTCAGCAGGTCAACGAGGACCACACGACCGT
TGAAGTCGTCTCCCGCCGCGGCAACGCCGTGATCATGTCCAAGGACGACTTCGACGCGCTGACCGAGACTGCCTACCTCC
TGCGCAACGCTGCGAACGCGGAGCGACTACTGGAGGCCGTCGAGCGTGCGCGTCGCGGAGAGTTTGAGCAGCACGACCTC
CTCGACGCGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT150509 WP_001261278.1 NZ_CP049102:140834-141064 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT150509 NZ_CP064760:c1252454-1252194 [Microbacterium schleiferi]
GTGACGCGATCGCTTTCCTTCGACCCGAACGGGTGGGAGGACTACGTCTACTGGCAGACACAGGACCGCCGGACGCTGAA
CCGGATCAACCTCCTCATCGCGGATATCGTTCGCGATCCGTTCGACGGCATCGGAAAGCCCGAACCGCTCCGTCACATAC
TTTCAGGCGCCTGGTCTCGTCGCATCGACGAGAAGAACCGACTCGTCTACTACGTGACCGATGAACACATTGTCATCCTG
CAGGCGCGAGACCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References