Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 136344..136869 Replicon plasmid p2
Accession NZ_CP049102
Organism Escherichia coli strain EC28

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag G5V60_RS25050 Protein ID WP_001159868.1
Coordinates 136344..136649 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag G5V60_RS25055 Protein ID WP_000813634.1
Coordinates 136651..136869 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5V60_RS25035 132276..133442 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
G5V60_RS25040 134030..134785 - 756 WP_000852146.1 replication initiation protein RepE -
G5V60_RS25045 135537..136343 - 807 WP_000016982.1 site-specific integrase -
G5V60_RS25050 136344..136649 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
G5V60_RS25055 136651..136869 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
G5V60_RS25060 137437..137949 + 513 WP_000151784.1 hypothetical protein -
G5V60_RS25065 137983..139116 - 1134 WP_000545984.1 DUF3800 domain-containing protein -
G5V60_RS25070 139283..140056 - 774 WP_000905949.1 hypothetical protein -
G5V60_RS25075 140069..140569 - 501 WP_000528932.1 hypothetical protein -
G5V60_RS25080 140834..141064 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
G5V60_RS25085 141061..141477 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..168051 168051


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T150508 WP_001159868.1 NZ_CP049102:c136649-136344 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T150508 NZ_CP064760:c1252454-1252194 [Microbacterium schleiferi]
GTGACGCGATCGCTTTCCTTCGACCCGAACGGGTGGGAGGACTACGTCTACTGGCAGACACAGGACCGCCGGACGCTGAA
CCGGATCAACCTCCTCATCGCGGATATCGTTCGCGATCCGTTCGACGGCATCGGAAAGCCCGAACCGCTCCGTCACATAC
TTTCAGGCGCCTGGTCTCGTCGCATCGACGAGAAGAACCGACTCGTCTACTACGTGACCGATGAACACATTGTCATCCTG
CAGGCGCGAGACCACTACTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT150508 WP_000813634.1 NZ_CP049102:c136869-136651 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT150508 NZ_CP064760:c1252702-1252451 [Microbacterium schleiferi]
ATGGCCGCCATCACCGCGACCGAAGCACGAAAGAGTCTGTTCGGGCTCATTCAGCAGGTCAACGAGGACCACACGACCGT
TGAAGTCGTCTCCCGCCGCGGCAACGCCGTGATCATGTCCAAGGACGACTTCGACGCGCTGACCGAGACTGCCTACCTCC
TGCGCAACGCTGCGAACGCGGAGCGACTACTGGAGGCCGTCGAGCGTGCGCGTCGCGGAGAGTTTGAGCAGCACGACCTC
CTCGACGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References