Detailed information of TA system TA150503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 91046..91671
• Replicon: plasmid p2
• Accession: NZ_CP049102
• Organism: Escherichia coli strain EC28

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G5V60_RS24750
• Protein ID: WP_000911313.1
• Coordinates: 91273..91671 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: G5V60_RS24745
• Protein ID: WP_000450520.1
• Coordinates: 91046..91273 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5V60_RS24745	91046..91273	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
G5V60_RS24750	91273..91671	+	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
G5V60_RS24755	91680..93833	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
G5V60_RS24760	94086..94817	-	732	WP_023565183.1	complement resistance protein TraT	-
G5V60_RS24765	94849..95346	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..168051	168051

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T150503 WP_000911313.1 NZ_CP049102:91273-91671 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T150503 NZ_CP064760:c483053-482646 [Microbacterium schleiferi]
GTGATCGTCGACACGAGCGCACTCCTCGCTTACTTCGACGCGAACGAGCCGCGCCACACCGATGTGGCTCGCATCATTGA
GAACACGGACGCCCCGCTCATCGTCTCGCCGTACGTGATCGCCGAACTCGACTACCTTGTCCTCACCCGACACGGGTCGG
ATGCCGAACTCGTCGTGCTCGACGAACTCACCTCTGGCGCGTGGGAACTGGCCGACATGACCAGCGCACGCATCCGTGTC
GCCACAGAGATCGTGCGACGTTACGCAGACCAGCCGATCGGCCTCACGGATGCCTCCAACATCGTGCTCGCAGACGCCTA
CCGCACCCGGCAGATCGCGACCCTCGATCGGCGGCACTTCGAGGTCTTGCGCTTCGACGACCACACCGCCATCGAGATCC
TCCCGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT150503 WP_000450520.1 NZ_CP049102:91046-91273 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT150503 NZ_CP064760:c483262-483050 [Microbacterium schleiferi]
ATGGTGAAGACCACGCTGTACCTCCCCGACGATCTGAAGCGTGCCATCGAGCTCGAAGCCGCGCGTCAGGGCAGCTCAGA
AGCTGACGTCATTCGCGAGACTCTTCGCCACGGCCTTTCGCAGACTCCCCGCGCGCCCCGCGGTGCCATCTTCACGGGCA
GCGAACCGATCGCCGAACGCGCCGATGAACTCCTCGCAGGGTTCGGCGAGTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2