Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4468957..4469454 Replicon chromosome
Accession NZ_CP049101
Organism Escherichia coli strain EC28

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G5V60_RS21875 Protein ID WP_001173343.1
Coordinates 4468957..4469130 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag G5V60_RS21880 Protein ID WP_001312331.1
Coordinates 4469158..4469454 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5V60_RS21865 4465627..4467084 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
G5V60_RS21870 4467321..4468838 + 1518 WP_164699417.1 lysine--tRNA ligase -
G5V60_RS21875 4468957..4469130 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G5V60_RS21880 4469158..4469454 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G5V60_RS21885 4469682..4469954 - 273 WP_000405647.1 N-acetyltransferase -
G5V60_RS21890 4470168..4474097 - 3930 WP_001528156.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4468957..4485200 16243


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T150498 WP_001173343.1 NZ_CP049101:c4469130-4468957 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T150498 NZ_CP064704:5900412-5900837 [Bradyrhizobium sp. 155]
GTGAATCTACTGCTCGACACCAACGTGTTGTCGGAGGTCCAGAGGCCCGCCCCTTCGCCGAAAGTCTTGTCATGGCTTGA
TACGATCGACGAGGATCGTGCGTTCATCAGCGCGGCATCGATCGCCGAGCTTCGTCGCGGCATCGCGTTGCTGGAGGACG
GCCGTCGCCGTGCAGCACTGGCCGCCTGGCTTGCCCGCGATCTGCCGGAGCGTTTTGCCGGGCGCACACTGCCGATCGAC
CACGCGGTCGCGGAACGCTGGGGTGACCTGATGGCTCAGAGTCGCAGAAGCGGTCTCGCATTGTCTGTCATGGACGGCTT
CTTTGCCGCGACCGCGCTGGCAAACAACCTCATGCTCGTCACGCGCAATGTGAAGGATTTTGCGGTATTCGGCGTGCCGC
TGCTGAACCCGTGGGACAACCCATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT150498 WP_001312331.1 NZ_CP049101:c4469454-4469158 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT150498 NZ_CP064704:5900137-5900415 [Bradyrhizobium sp. 155]
ATGACGGAACACACTGCACCAGATTCACCAGCGACGGACTCGTGGACGCTTGCCAACGCCAAGGCCCGGCTCTCCGAGGT
GATCGATCGTGCCCAAGCCGGCCCGCAAATCATCACCCGACACGGCAAGCCCAACGCGGTGATCGTTTCCGTCGAGGAAT
GGGCGCGCAAGACGGCGCGCAAAGGTACCTTGGCCGAATTCCTGCTGGCTTCGCCGCTGCGCAGCGGCGACCTCGAGCTT
GAACGCGTTCACGACGCGCCGCACGACGAGCTGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References