Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4076718..4077268 Replicon chromosome
Accession NZ_CP049101
Organism Escherichia coli strain EC28

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag G5V60_RS19925 Protein ID WP_001160966.1
Coordinates 4076718..4077032 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag G5V60_RS19930 Protein ID WP_000125566.1
Coordinates 4077035..4077268 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5V60_RS19900 4072393..4073679 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
G5V60_RS19905 4073679..4074668 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
G5V60_RS19910 4074665..4075486 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
G5V60_RS19915 4075489..4075866 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
G5V60_RS19920 4075873..4076721 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
G5V60_RS19925 4076718..4077032 - 315 WP_001160966.1 CcdB family protein Toxin
G5V60_RS19930 4077035..4077268 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
G5V60_RS19935 4077354..4077833 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
G5V60_RS19940 4078025..4079887 - 1863 WP_001523933.1 glutathione-regulated potassium-efflux system protein KefC -
G5V60_RS19945 4079880..4080410 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
G5V60_RS19950 4080518..4081849 - 1332 WP_001523932.1 MFS transporter -
G5V60_RS19955 4081908..4082195 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T150497 WP_001160966.1 NZ_CP049101:c4077032-4076718 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T150497 NZ_CP064704:3757114-3757509 [Bradyrhizobium sp. 155]
ATGGTGCTGGATACGTCTGCGATCGTCGCGACCGTCACCAACGAGCCAGATGGGCAGCGCTATCGGAAAGCCATGCTGGA
TGCTGCCAGCCTGTCGATGTCAGCAGTAGCAATCCTGGAAACCAAGATCGTTCTCTCTGCTCGCTTCGGAGCATCGGCCG
TGAAGTTCTTCGACGAATTGCTGGAGATTGCCGGGATTGTCGTAGTGCCGTTCGACGCAGACATGGCGAAAGGCGCGTTC
GAGGCATTTCAACGCTTCGGCAAGGGTCGGGGGACATCCGGCGCAGCTCAACATCGTCGATTGTGCGGTCTAGGCTTGGC
GAAAGCGCGTTCGCAACCGCTGCTCTTCAAGGGAGCCCACTTTGCTAAGACAGACATTCTCCCCGCGTTGACCTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT150497 WP_000125566.1 NZ_CP049101:c4077268-4077035 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT150497 NZ_CP064704:3756872-3757114 [Bradyrhizobium sp. 155]
ATGAACATCCGTTCCGCGAAAGTAGATGAGCTTGCCCAGCGCCTCGCCCGCCTGACGGGGGAAGATATCGAGACGGCGCT
CGAACGCGCCATCGAGGAGCGCTTGTCTCGCGTGGCATCGCCGGCGGTCGATAGGAGGGCCGCCCTGACGTCGTTCTTCG
AGCGAACCGCTGGCTTGCCCGTTCTCGATTCTCGGCCTGCAGACGAAATCCTCGGCTATGACCGTTTCGGGCTGCCGTCC
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References