Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3645476..3646094 Replicon chromosome
Accession NZ_CP049101
Organism Escherichia coli strain EC28

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag G5V60_RS17955 Protein ID WP_001291435.1
Coordinates 3645876..3646094 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag G5V60_RS17950 Protein ID WP_000344800.1
Coordinates 3645476..3645850 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5V60_RS17940 3640566..3641759 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
G5V60_RS17945 3641782..3644931 + 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
G5V60_RS17950 3645476..3645850 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
G5V60_RS17955 3645876..3646094 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
G5V60_RS17960 3646268..3646819 + 552 WP_000102539.1 maltose O-acetyltransferase -
G5V60_RS17965 3646935..3647405 + 471 WP_000136192.1 YlaC family protein -
G5V60_RS17970 3647569..3649119 + 1551 WP_001524176.1 cyclic-guanylate-specific phosphodiesterase PdeB -
G5V60_RS17975 3649161..3649514 - 354 WP_000878140.1 DUF1428 domain-containing protein -
G5V60_RS17985 3649893..3650204 + 312 WP_000409908.1 MGMT family protein -
G5V60_RS17990 3650235..3650807 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T150496 WP_001291435.1 NZ_CP049101:3645876-3646094 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T150496 NZ_CP064703:7765745-7766011 [Bradyrhizobium sp. 170]
ATGAAAACCGCTTGGACGGAAGATGCCTGGCAAGACTATCTGCAATGGCAAAAGGAGGATGAAAAGATCCTCGAAGTCAT
AAACGGTCTAATCAAAGATATTAAGCGCGATCCGTTCAAGGGGCTTGGGAAACCAGAGCCCCTTAAACATGCGCTCCAGG
GATGGTGGTCGCGCCGCATTTCAGGCGAACATCGCTTGGTGTATCGCGTTTCTGGAAAGGGTGACACTCAGCAACTCGAC
ATTGCTCAGTGCCGCTATCATTACTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT150496 WP_000344800.1 NZ_CP049101:3645476-3645850 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT150496 NZ_CP064703:7765470-7765748 [Bradyrhizobium sp. 170]
ATGGCACACGTTTCTTACACTGAACTGCGTAACAACCTCGCGTCCTACATGGACGAGGTTTGCGATGATCGCGCCCCCCT
GTTCGTCACCCGCCAGAATGCCCGCACCGTCGTTCTCATGGCCGAGGATGAGTATGAGAGTCTCATGGAGACCGTCCACC
TCCTGAAGAGTCCCGCCAATGCAGTACGTCTTCTACGTTCCATCAAGGAAGCGGACGAGGGCAAACTAACTGAACGCGAG
TTGATCGAGCCAACAAAGGCAGGTTCGGCCAAGGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References