Detailed information of TA system TA150493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2524977..2526565
• Replicon: chromosome
• Accession: NZ_CP049101
• Organism: Escherichia coli strain EC28

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: G5V60_RS12290
• Protein ID: WP_021540355.1
• Coordinates: 2525243..2526565 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A061YNW4
• Locus tag: G5V60_RS12285
• Protein ID: WP_001296076.1
• Coordinates: 2524977..2525243 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5V60_RS12270	2521345..2522796	+	1452	WP_000854637.1	tagaturonate reductase	-
G5V60_RS12275	2522993..2523907	+	915	WP_001385356.1	bestrophin family protein	-
G5V60_RS12280	2523911..2524669	-	759	WP_045132635.1	trans-aconitate 2-methyltransferase	-
G5V60_RS12285	2524977..2525243	+	267	WP_001296076.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
G5V60_RS12290	2525243..2526565	+	1323	WP_021540355.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
G5V60_RS12295	2527477..2528040	+	564	WP_001296074.1	type 1 fimbrial major subunit FimA	-
G5V60_RS12300	2528402..2529112	+	711	WP_021576179.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49271.92 Da
• Isoelectric Point: 8.1297

>T150493 WP_021540355.1 NZ_CP049101:2525243-2526565 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T150493 NZ_CP064703:283861-284265 [Bradyrhizobium sp. 170]
GTGAAGGCGTTTTTCGATACCAACATTCTCGTCTACACCGCGACTTCGGACGCCAGAAAACAACGGGCGGTGGATTGCCT
GAGCCGCGGCGGTGTCGTCAGCGTTCAGGTGCTGAACGAGTTCGTTCACGTCGCCCGCCGCAAGCTTCGTCATGACTGGC
CGCAGATCGAACTGGCGCTCGGGCTGTTCCGCGCGTCGCTCGATGACGTCTTGCCCGTCACGCTGAACATTCACACGGGC
GCCGTGTCCCTCGCCCGCGAGCACGGCGTGCCGTTCTATGACGCCCTGATCGTCGCCGCCGCCATCGAGGCCGGTTGTGA
CACGCTGTTCAGCGAAGACATGCAGCACGGCCGCAGCATCGGCGGCCTCGCCATCGTCAATCCGTTTCTCGATAGTGCAC
CGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9987.34 Da
• Isoelectric Point: 4.9632

>AT150493 WP_001296076.1 NZ_CP049101:2524977-2525243 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT150493 NZ_CP064703:283634-283864 [Bradyrhizobium sp. 170]
ATGCAGGTTTCAAAATGGGGTGACAGCCTGGCCGTGCGGCTGCCCAAAGCCCTGGTCGAAAAGATGGGCCTGCGGGCAGG
CGACGAACTCGACATCGTCGACGTGGCCGAGCGAACGCTGATCGTCCAGAAGGAAGACCGCCGCAAGGCCGCGCTCGAGC
GTCTGGCTTCGCTGAACTGGGCGCTTCCTCCCGATTACAAGTTCGATCGGGACGAGGCGAACGAACGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A061YNW4