Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2015682..2016272 Replicon chromosome
Accession NZ_CP049101
Organism Escherichia coli strain EC28

Toxin (Protein)


Gene name pemK Uniprot ID V0VE57
Locus tag G5V60_RS09620 Protein ID WP_000373724.1
Coordinates 2015940..2016272 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag G5V60_RS09615 Protein ID WP_000288812.1
Coordinates 2015682..2015939 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5V60_RS25685 2014366..2014752 + 387 WP_187415326.1 ash family protein -
G5V60_RS09600 2014664..2015077 + 414 WP_001527191.1 hypothetical protein -
G5V60_RS09605 2015128..2015334 + 207 WP_001527190.1 helix-turn-helix domain-containing protein -
G5V60_RS25690 2015393..2015584 - 192 WP_077561361.1 hypothetical protein -
G5V60_RS09615 2015682..2015939 + 258 WP_000288812.1 antitoxin Antitoxin
G5V60_RS09620 2015940..2016272 + 333 WP_000373724.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
G5V60_RS09625 2016319..2016675 - 357 WP_024186914.1 hypothetical protein -
G5V60_RS09630 2016656..2016982 - 327 Protein_1878 hypothetical protein -
G5V60_RS09635 2017120..2017998 + 879 WP_001527182.1 integrase domain-containing protein -
G5V60_RS09640 2018836..2020098 - 1263 WP_001527179.1 integrase arm-type DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1989717..2030921 41204


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11724.57 Da        Isoelectric Point: 10.5834

>T150484 WP_000373724.1 NZ_CP049101:2015940-2016272 [Escherichia coli]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T150484 NZ_CP064692:c3072122-3071202 [Bradyrhizobium sp. 191]
ATGGCGCGTGAGCCGCGCAACATCGGCGCATCAGTGCGAGCGCGACTCCTCGATCGGGCACGTGCCGAACGGTCGGATTT
CCAGATTCTGCTGACGCGCTATGCGCTTGAGCGTCTGCTCTATCGGCTGAGCGTGTCACCACACCGCGACCGCTTCATCC
TCAAAGGTGCGATGCTCTTCGTGACCTGGGTCGCCGATCCGTTTCGGCCTACGCGCGATCTGGACCTGCTTGGCAATGGC
GACAGCGACGCCGAGGCCATTGCCGAAACCTTCCGGGCCATTTGTGCGCAACCCGTCGCCGACGACGGGGTGGCGTTCGA
TGTCGCCGCGCTGATGGCGGCCCCGATCCGTGAGGAGGTGGAATATGGTGGCGTGCGGGTCCGCACAACGGCAACCATCG
CCGGCGCGCGTATTCCGATCCAGGTCGACATTGGTTTCGGCGATGCCATTACACCCGCGGCGGTCGAGATCGACTATCCC
ACCCTGCTGGACGCCCCAACGCCGCACCTTCGCGCATATCCGGTCGAAACGGTGGTGGCCGAGAAATTCGAAGCGCTTGT
AACGCTCGGCGTGGCCAATAGCCGGCTCAAGGATTTCTATGATCTTTGGGTGATCTCCCGAACATTCGAGCTTCGCCGGG
CTGCCCTTGTCGAGGCAATTCAGCGGACCTTCGAAAGACGCGGAACGGTCCTCCCATCCGTTGTGCCGGTGGGTCTGACC
GATGAATTTGCAGAAGCATCGGCCGCTCAGTGGCGCGCCTTCCTAGGCCGCGACCGTATGGCAGCAGCACCAGACGTTTT
CGCCATTGCCGTCGCCGATCTTCGCCTCTTCTTGATGCCGCTGGTCGTCGGCTTGGAGGAAGAACGAATTTGGCCGCCGA
GCGGGCCGTGGTCGCCGGGAGCAGCAATCGATGAGGCGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT150484 WP_000288812.1 NZ_CP049101:2015682-2015939 [Escherichia coli]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT150484 NZ_CP064692:c3072732-3072112 [Bradyrhizobium sp. 191]
ATGCCATCCACCAGCGGCCAGCGGCTTCGTGCGCTCGATCTCCTGAAGACCCGGGGAATGCTCCGGCTGAAGGACTTCGT
CGCCGAAGGCATTGGCCCGGAGACACTGGCCCGTCTTGTCAGGGAGGAGGTAGTCGTCCGCCCGGCACGTGGCCTCTACC
AACTCCCGGACGCCCACGTCGAGGCTGCTCATACACTGGCTGAGGCGGCGGTTCTGGTTCCAAAGGGTGTAGTCTGCCTG
ACCTCAGCCCTCCAATACCACGAACTGACGTTGCAGATGCCATCGGCTGTATGGATGGCGATCGACCGTGTCGCCTGGCG
GCCCAAGATCGATTATCCCGCTATTCGATTCGTGCGCTTCACTGGATCGGCTCTGACCGAAGGAGTTGAGCGTCACCGCA
TCGAGAATGTTGACGTTCCCATCACGAATCCGGCGAAGACAATTGTCGATTGTTTTCGCTACCGGACCAAGATTGGCCTC
GACGTCGCGATGGAGGGGCTGCGCGAGGGTCTCCGCCGTCGCCGATGCAAGCCGGATCAGCTCTGGCGTTACGCGCGCAA
GGCGCGGGTCTGGTCGGTCATGCGCCCTTACGTCGAAGCAATGGTGTCCGATGGCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VE57

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References