Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 970643..971297 Replicon chromosome
Accession NZ_CP049101
Organism Escherichia coli strain EC28

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag G5V60_RS04740 Protein ID WP_000244765.1
Coordinates 970890..971297 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G5V60_RS04735 Protein ID WP_000354046.1
Coordinates 970643..970909 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5V60_RS04715 966731..968164 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
G5V60_RS04720 968209..968520 + 312 WP_001525592.1 N(4)-acetylcytidine aminohydrolase -
G5V60_RS04725 968684..969343 + 660 WP_000250274.1 hemolysin III family protein -
G5V60_RS04730 969420..970400 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
G5V60_RS04735 970643..970909 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G5V60_RS04740 970890..971297 + 408 WP_000244765.1 protein YgfX Toxin
G5V60_RS04745 971337..971858 - 522 WP_001055874.1 flavodoxin FldB -
G5V60_RS04750 971970..972866 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G5V60_RS04755 972891..973601 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G5V60_RS04760 973607..975340 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T150482 WP_000244765.1 NZ_CP049101:970890-971297 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T150482 NZ_CP064692:2525071-2525496 [Bradyrhizobium sp. 191]
GTGAATCTGCTGCTCGACACCAACGTGCTGTCGGAGGTTCGGCGCTCAGCGCCTTCGCCGAAGGTCTTGGCGTGGCTCGA
TACGATCGACGAAGATCGGGCATTCATAAGCGTTGCCACGATCGCCGAACTTCGCCGCGGCATCGCATTGCTGGACGATG
GCCGCCGGCGCACGGCGCTGGCCGCCTGGCTGGCGCATGATCTACCGGCGCGATTTGCCGACCGCGTTCTGCCCATCGAC
CATGCGGTCGCCGAGCGCTGGGGCGACCTCATGGCGCAGAGCCGCATGGGCGGCGTCGCATTGTCTGTCATGGACGGCTT
CTTTGCGGCCACCACTCTCGTGCACCACCTCACGCTTGTCACGCGCAATGTGAAGGATTTTGCGGCGTTCGGCGTTCCGC
TGCTCAATCCTTGGGATGATGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT150482 WP_000354046.1 NZ_CP049101:970643-970909 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT150482 NZ_CP064692:2524790-2525074 [Bradyrhizobium sp. 191]
ATGACCGATCGTATTACTGCGCCCCGCACCCTTCCTGCTGATGACACCTGGACGCTGGCCAGCGCCAAGGCGCGGTTGTC
CGAGGTGATTGATCGCGCTCAAGCCGGCCCGCAGGTCATCACACGACACGGCAAACCGAATGCGGTTGTCGTCTCCGCCG
ACGAATGGGCGCGCAAGACCGCACGCAACGGCACGCTGGCCGAATTCCTTCTGGGCTCGCCGCTGCGCGGCGCCAACCTC
GAGCTTGAACGGGTCCAGGACGCGCCACGCGACGAGATGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References