Detailed information of TA system TA150481

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 836736..837571
• Replicon: chromosome
• Accession: NZ_CP049101
• Organism: Escherichia coli strain EC28

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: G5V60_RS04040
• Protein ID: WP_001683029.1
• Coordinates: 836736..837113 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: G5V60_RS04045
• Protein ID: WP_001278232.1
• Coordinates: 837203..837571 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5V60_RS04015	832809..833792	-	984	WP_001527653.1	KpsF/GutQ family sugar-phosphate isomerase	-
G5V60_RS04020	834603..834773	-	171	Protein_789	IS110 family transposase	-
G5V60_RS04025	835115..835957	-	843	WP_001519083.1	DUF4942 domain-containing protein	-
G5V60_RS04030	836042..836239	-	198	WP_001519084.1	DUF957 domain-containing protein	-
G5V60_RS04035	836251..836739	-	489	WP_001519085.1	hypothetical protein	-
G5V60_RS04040	836736..837113	-	378	WP_001683029.1	TA system toxin CbtA family protein	Toxin
G5V60_RS04045	837203..837571	-	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
G5V60_RS04050	837734..837955	-	222	WP_000692315.1	DUF987 domain-containing protein	-
G5V60_RS04055	838024..838500	-	477	WP_001186727.1	RadC family protein	-
G5V60_RS04060	838516..838995	-	480	WP_019842849.1	antirestriction protein	-
G5V60_RS04070	839334..840152	-	819	WP_021524998.1	DUF945 domain-containing protein	-
G5V60_RS04075	840242..840475	-	234	WP_001278282.1	DUF905 family protein	-
G5V60_RS04080	840481..841158	-	678	WP_001519094.1	hypothetical protein	-
G5V60_RS04085	841306..841986	-	681	WP_001282920.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14347.36 Da
• Isoelectric Point: 8.7451

>T150481 WP_001683029.1 NZ_CP049101:c837113-836736 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T150481 NZ_CP064692:1853367-1853774 [Bradyrhizobium sp. 191]
ATGACGAAATGGCTTCTCGACACCAACGCCGTCATCGCACTCGTCACACGCCGGTCAGAGCCTCTCCTGCGCCGAGTCGA
ATCCAGCGAGCCCGGTGCACTCGCGATCTCGGCCATCGTTACCCACGAACTCTACTTCGGCGCCTATCGCAGCCAGAAAG
TCGAATTCAATCTGGAAACACTCCGTCTTCTCTTTGCCGATCTCGAAATCCTCGATCTTGACCAAGAAGACGCGCGAGCC
ACCGGCGAGATCCGGGCGGACCTTGCTCGGCGCGGCACGCCGATCGGCCCCTATGACCTCCTGATCGCCGGCCAAGCGAT
CGCGCGCGGCCTGCCGCTTGTCAGCAACAACACCGCCGAATTCCAGCGCATTGCCGGATTGCGACTGGAGGACTGGACGA
GGGATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT150481 WP_001278232.1 NZ_CP049101:c837571-837203 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT150481 NZ_CP064692:1853110-1853370 [Bradyrhizobium sp. 191]
ATGTCCCACATTGCGAAGGTGTTCAAATCGGGCAACTCCCAGGCCGTCCGGCTGCCGAAGGATTTTCGTTTCTCGGTCGA
TGAAGTCGAAGTGTCCCGCGAAGGCGAGGCTGTGATCCTGCGCCCAAGGCGGAGCGCGACCGGCCCGTGGACATCGCTTC
GCGCGGCCGTCGCACGCGGTTTCAGCCCGGACTTCATGGCTGAAGGGCGGGAGCAACCTGAAGAGCAGACGCGTCCCGAT
CTGAACCAGGCGTTTCGATGA

Structures

Antitoxin

• AlphaFold DB: E3PJ72