Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 311171..311971 Replicon chromosome
Accession NZ_CP049101
Organism Escherichia coli strain EC28

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag G5V60_RS01445 Protein ID WP_000342452.1
Coordinates 311444..311971 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag G5V60_RS01440 Protein ID WP_001277107.1
Coordinates 311171..311437 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5V60_RS01420 306830..307498 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
G5V60_RS01425 307491..308549 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
G5V60_RS01430 308794..309648 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
G5V60_RS01435 309919..311022 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
G5V60_RS01440 311171..311437 + 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
G5V60_RS01445 311444..311971 + 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
G5V60_RS01450 311968..312351 - 384 WP_001525853.1 aspartate 1-decarboxylase autocleavage activator PanM -
G5V60_RS01455 312774..313883 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
G5V60_RS01460 313931..314857 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
G5V60_RS01465 314854..316131 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
G5V60_RS01470 316128..316895 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T150479 WP_000342452.1 NZ_CP049101:311444-311971 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T150479 NZ_CP064690:c5421956-5421690 [Bradyrhizobium sp. 192]
ATGGCGCACGTTTCGTACACGGAGCTGCGGGGAAATCTTGCGGCCTACATGGATCAGGTCTGCGACGATCGCGCGCCTCT
CGTTGTGACGCGCCAGAACGCCCGCAGCGTGGTGATGCTCTCGGAGGAAGAGTATGAGGGTCTTGTGGAGACGGTCCATC
TTCTGAAGAGCCCGGCCAATGCCGCACGCCTTCTGCGCTCCATCAAGGAGGCCGACGAAGGCAAGCTCGTCGCGCATGAA
TTGATCGAGCCCGGCTCGACCGGCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT150479 WP_001277107.1 NZ_CP049101:311171-311437 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT150479 NZ_CP064690:c5421684-5421418 [Bradyrhizobium sp. 192]
ATGCTGATCGCCTGGACCGAGATCGCCTGGCAAGACTACCTGCGATGGCAGCAAGAGGACGAAAAAATCCTCTCGGCCAT
CAACGATCTGATCAAGGACATTAAGAGAGATCCGTTCAAGGGGATCGGAAAGCCGGAGCCCCTCAAACATGATCTGCGCG
GCTGGTGGTCTCGCCGCATCACGGGCGAACACCGGCTGGTGTATCGCCTTCAGGGCAAGGATGACGCGCAACAACTCGTC
ATCGCCCAATGTCGATACCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References