Detailed information of TA system TA150463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 255717..257404
• Replicon: chromosome
• Accession: NZ_CP049090
• Organism: Glaesserella parasuis strain aHPS7

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: G5S31_RS01320
• Protein ID: WP_160404948.1
• Coordinates: 255717..257060 (+)
• Length: 448 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: G5S31_RS01325
• Protein ID: WP_015940035.1
• Coordinates: 257060..257404 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S31_RS01295	251686..252309	-	624	WP_160404946.1	TonB-dependent receptor	-
G5S31_RS11505	252328..252528	-	201	WP_021118337.1	TonB-dependent receptor	-
G5S31_RS01300	252538..252762	+	225	Protein_253	hypothetical protein	-
G5S31_RS01305	253005..254684	+	1680	WP_005713398.1	DNA repair protein RecN	-
G5S31_RS01310	254764..254991	-	228	Protein_255	transposase	-
G5S31_RS01315	255166..255603	+	438	WP_062924029.1	ribosomal protein S18-alanine N-acetyltransferase	-
G5S31_RS01320	255717..257060	+	1344	WP_160404948.1	HipA domain-containing protein	Toxin
G5S31_RS01325	257060..257404	+	345	WP_015940035.1	helix-turn-helix domain-containing protein	Antitoxin
G5S31_RS01330	257433..258758	+	1326	WP_021111225.1	ATP-dependent RNA helicase SrmB	-
G5S31_RS01335	258879..259511	+	633	WP_015940037.1	lipoprotein insertase outer membrane protein LolB	-
G5S31_RS01340	259513..260358	+	846	WP_021113204.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
G5S31_RS01345	260386..261336	+	951	WP_005713153.1	ribose-phosphate pyrophosphokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	254764..255015	251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (161-407)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 448 a.a.
• Molecular weight: 51136.23 Da
• Isoelectric Point: 5.4867

>T150463 WP_160404948.1 NZ_CP049090:255717-257060 [Glaesserella parasuis]
MQIEPLILQAYIADRWQDIAILTFQADYSLNDFSYLHDLTEILSDNDKAVSFNYPVEFFPHRSVSGWFAFLDDIVPSGAS
RRYWLQKLDIAHLPMAQQNYLLLKQGTIAPVGHLRVKQAVSSFVENEPSRLFEIDDVINRQADFLEYANQRGAMAGGATG
AGGEAPKLLLRQTATRQVWIDNLQQGASSDDFYLVKFPRGKRSVIDCDILRTEFHYYHELASMGFDTIDTTKMRLEEGER
YPSLWLPRFDVYRNEQGKMARYAVESVYAMLEKGAGSLLDHEKVIRQLIAKIETSHLVETGMFFDKQAFVIEWVRRDLLN
IAFGNSDNHGRNTAFLRNQNRIWLAPIYDFAPMKADPEGIPRTTMWDSKGHPALELGGEYRFDLIAEQLDDLCPADKLLD
ALRQTAQQLVGLKARLEARGVPTAILTYPSISLDYLPEKLKRWQLLS

Nucleotide

• Length: 1344 bp

>T150463 NZ_CP064682:4438509-4438859 [Escherichia coli K-12]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13225.52 Da
• Isoelectric Point: 10.2782

>AT150463 WP_015940035.1 NZ_CP049090:257060-257404 [Glaesserella parasuis]
MEEIKQARASKSKKTLDIYQRATVRDEIARKLLLNEMSLGQALKYLRLHLLAMKQERYAEIVKVSRKTLSDLENDKGNYS
IDIINQVLRPFELQLGVVPMNKTLLRQVLNEQAV

Nucleotide

• Length: 345 bp

>AT150463 NZ_CP064682:4438264-4438515 [Escherichia coli K-12]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA