Detailed information of TA system TA150453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 872139..872916
• Replicon: chromosome
• Accession: NZ_CP049089
• Organism: Glaesserella parasuis strain vHPS7

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: G5C67_RS04490
• Protein ID: WP_075630679.1
• Coordinates: 872139..872639 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: U4SPD7
• Locus tag: G5C67_RS04495
• Protein ID: WP_005710653.1
• Coordinates: 872629..872916 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5C67_RS04455	867265..867420	-	156	WP_021116533.1	YoaH family protein	-
G5C67_RS04460	867438..868166	-	729	WP_021112446.1	tRNA pseudouridine(65) synthase TruC	-
G5C67_RS04465	868163..868477	-	315	WP_005710664.1	YqcC family protein	-
G5C67_RS04470	868519..869433	-	915	WP_021113663.1	N-acetylmuramic acid 6-phosphate etherase	-
G5C67_RS04475	869445..870560	-	1116	WP_016527792.1	anhydro-N-acetylmuramic acid kinase	-
G5C67_RS04480	870714..871049	-	336	WP_016527793.1	outer membrane protein assembly factor BamE	-
G5C67_RS04485	871110..872111	-	1002	WP_010786647.1	nucleoid-associated protein YejK	-
G5C67_RS04490	872139..872639	-	501	WP_075630679.1	GNAT family N-acetyltransferase	Toxin
G5C67_RS04495	872629..872916	-	288	WP_005710653.1	DUF1778 domain-containing protein	Antitoxin
G5C67_RS04500	873062..873451	+	390	WP_075630680.1	RidA family protein	-
G5C67_RS04505	873455..874192	+	738	WP_021112465.1	peptidoglycan editing factor PgeF	-
G5C67_RS04510	874367..875944	+	1578	WP_005710646.1	FAD-binding protein	-
G5C67_RS04515	876048..876548	+	501	WP_016527798.1	CvpA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (4-87)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 19147.32 Da
• Isoelectric Point: 9.2835

>T150453 WP_075630679.1 NZ_CP049089:c872639-872139 [Glaesserella parasuis]
MKSKFIEEPLTKHHQREAFDCGNLEMNRFFKQYSRQSHEKGTSKTYISRHLDSQQIIGFYTITLSALDQQHLPELCKKRF
GYYPIPLFTLARLAVDIRYQKQGIGGMLLVKAFKRCAIVAEQVGGIGLLIEAKDEDISRWYQSYGAIPLENMLLTLILLF
DTIKGL

Nucleotide

• Length: 501 bp

>T150453 NZ_CP064682:c2083194-2082943 [Escherichia coli K-12]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10917.47 Da
• Isoelectric Point: 6.4650

>AT150453 WP_005710653.1 NZ_CP049089:c872916-872629 [Glaesserella parasuis]
MPQIANALNDRMSIRISTEDKQTLIRAVALTHTNITEFVLQRILPSAREIIQQYDNRQFSPQDLSLIMELLDNPPKANTK
LQQAAKLAKESYHEE

Nucleotide

• Length: 288 bp

>AT150453 NZ_CP064682:c2082946-2082692 [Escherichia coli K-12]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: U4SPD7