Detailed information of TA system TA150451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1888359..1890046
• Replicon: chromosome
• Accession: NZ_CP049088
• Organism: Glaesserella parasuis strain sHPS7

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: G5C59_RS09260
• Protein ID: WP_021118651.1
• Coordinates: 1888359..1889702 (+)
• Length: 448 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: G5C59_RS09265
• Protein ID: WP_015940035.1
• Coordinates: 1889702..1890046 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5C59_RS09235	1884329..1884952	-	624	WP_021118336.1	TonB-dependent receptor	-
G5C59_RS09240	1885181..1885405	+	225	Protein_1800	hypothetical protein	-
G5C59_RS09245	1885648..1887327	+	1680	WP_005713398.1	DNA repair protein RecN	-
G5C59_RS09250	1887407..1887634	-	228	Protein_1802	transposase	-
G5C59_RS09255	1887808..1888245	+	438	WP_021118650.1	ribosomal protein S18-alanine N-acetyltransferase	-
G5C59_RS09260	1888359..1889702	+	1344	WP_021118651.1	type II toxin-antitoxin system HipA family toxin	Toxin
G5C59_RS09265	1889702..1890046	+	345	WP_015940035.1	helix-turn-helix domain-containing protein	Antitoxin
G5C59_RS09270	1890075..1891400	+	1326	WP_021118652.1	ATP-dependent RNA helicase SrmB	-
G5C59_RS09275	1891521..1892153	+	633	WP_021110345.1	lipoprotein insertase outer membrane protein LolB	-
G5C59_RS09280	1892155..1893000	+	846	WP_021113204.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
G5C59_RS09285	1893028..1893978	+	951	WP_021118653.1	ribose-phosphate pyrophosphokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (161-407)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 448 a.a.
• Molecular weight: 51033.02 Da
• Isoelectric Point: 5.4908

>T150451 WP_021118651.1 NZ_CP049088:1888359-1889702 [Glaesserella parasuis]
MQIEPLTLQAYIADRWQDIALLTFKADYSVNDFSYLHDLTEILSDNDKAVSFNYPVEFFPHRSVSGWFAFLDDIVPSGAS
RRYWLQKLDIAHLPMAQQNYLLLKQGTIAPVGHLRVKQAVSSFVENEPSRLFEIDDVINRQADFLEYANQRGAMAGGATG
AGGEAPKLLLRQTATRQVWIDNLQQGASSDDFYLVKFPRGKRSVIDCDILRAEFHYYHELASMGFDTIDTTKMRLEEGER
YPSLWLPRFDVYRNEQGKMARYAVESVYAMLEKGAGSLLDHEKVIRQLIAKIENSHLAETGMDFDKQAFVIEWVRRDLLN
IAFGNSDNHGRNTAFLRNQNRIWLAPIYDFAPMKADPEGIPRTTMWDSKGHPALELGGEYRFDLIAEQLDDLCPADKLLD
ALRQTAQQLVGLKARLEARGVPTAILTYPSISLDYLPEKLKRWQLLS

Nucleotide

• Length: 1344 bp

>T150451 NZ_CP064682:2071050-2071424 [Escherichia coli K-12]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13225.52 Da
• Isoelectric Point: 10.2782

>AT150451 WP_015940035.1 NZ_CP049088:1889702-1890046 [Glaesserella parasuis]
MEEIKQARASKSKKTLDIYQRATVRDEIARKLLLNEMSLGQALKYLRLHLLAMKQERYAEIVKVSRKTLSDLENDKGNYS
IDIINQVLRPFELQLGVVPMNKTLLRQVLNEQAV

Nucleotide

• Length: 345 bp

>AT150451 NZ_CP064682:2070593-2070961 [Escherichia coli K-12]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA