Detailed information of TA system TA150450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1369222..1370771
• Replicon: chromosome
• Accession: NZ_CP049088
• Organism: Glaesserella parasuis strain sHPS7

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: G5C59_RS06725
• Protein ID: WP_021118604.1
• Coordinates: 1369222..1370538 (-)
• Length: 439 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: U4SAZ6
• Locus tag: G5C59_RS06730
• Protein ID: WP_010786623.1
• Coordinates: 1370532..1370771 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5C59_RS06710	1366182..1367054	+	873	WP_021118607.1	LysR family transcriptional regulator	-
G5C59_RS06715	1367115..1367729	-	615	WP_021118606.1	DsbA family protein	-
G5C59_RS06720	1367953..1369218	+	1266	WP_206337548.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
G5C59_RS06725	1369222..1370538	-	1317	WP_021118604.1	type II toxin-antitoxin system HipA family toxin	Toxin
G5C59_RS06730	1370532..1370771	-	240	WP_010786623.1	helix-turn-helix domain-containing protein	Antitoxin
G5C59_RS06735	1370919..1371746	-	828	WP_021118603.1	alpha/beta hydrolase	-
G5C59_RS06740	1371877..1373322	+	1446	WP_164681687.1	metalloprotease TldD	-
G5C59_RS06745	1373576..1373986	+	411	WP_020457617.1	preprotein translocase subunit SecE	-
G5C59_RS06750	1373988..1374566	+	579	WP_020457616.1	transcription termination/antitermination protein NusG	-
G5C59_RS06755	1374676..1375646	+	971	Protein_1328	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1374676..1375188	512

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-104)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-66)

Sequences

Toxin

Protein

• Length: 439 a.a.
• Molecular weight: 50081.40 Da
• Isoelectric Point: 7.9228

>T150450 WP_021118604.1 NZ_CP049088:c1370538-1369222 [Glaesserella parasuis]
MVMRLRVLMNGEMVGYFDRTLSGSHHFTYAESWLNSPLARPISLSMPLQRATYQGKEVINFFDNLLPDNPQIRARIATRF
NAETQQPFDLLAKIGRDAAGAITLLGEDEQIPNHRIIQATELNESEMAKILQSYQYNVPLGMIDNNQDFRISIAGAQEKT
ALLWHNGKWKLPHGLTPTTHIFKLPMGEIRTPKHLLDLSKSVDNELVCLRLFSAFNIPSANVELLEMNDIRALAVERFDR
RLSQDGSYFLRLPYEDFCQAFGLSPTQKYESDGGIGIGDMMNKLLYSSNSNDRRQFMQTQFLFWLLAAIDGHAKNFSLAI
EKQGRYHLSPVYDVISAYPMLGGKGWHHRELQMAMSLRSTQKGKKWHWNKIYAEHFYATAKFVGFSSSDMKQVIEQICDT
VQETIDSVATTLPKQIDENVRDQIFTGIMKQVQKVSMK

Nucleotide

• Length: 1317 bp

>T150450 NZ_CP064682:c1917464-1917361 [Escherichia coli K-12]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9059.47 Da
• Isoelectric Point: 9.7684

>AT150450 WP_010786623.1 NZ_CP049088:c1370771-1370532 [Glaesserella parasuis]
MLITTPDMLGNSLRDIRKQKKLSQMQIANQVALRQATISTFEKKTDKATIETLFKLLSGLGYELHIQPRPEKTEGNDEW

Nucleotide

• Length: 240 bp

>AT150450 NZ_CP064682:1917323-1917594 [Escherichia coli K-12]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: U4SAZ6