Detailed information of TA system TA150447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 567395..568008
• Replicon: chromosome
• Accession: NZ_CP049088
• Organism: Glaesserella parasuis strain sHPS7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: G5C59_RS02845
• Protein ID: WP_200859958.1
• Coordinates: 567395..567562 (+)
• Length: 56 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1T0AB61
• Locus tag: G5C59_RS02850
• Protein ID: WP_005714429.1
• Coordinates: 567601..568008 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5C59_RS02800	562458..563003	-	546	WP_021118466.1	lysozyme	-
G5C59_RS02805	562978..563277	-	300	WP_206337554.1	hypothetical protein	-
G5C59_RS02810	563389..563838	+	450	WP_042905676.1	hypothetical protein	-
G5C59_RS02815	563801..563995	-	195	WP_021110729.1	hypothetical protein	-
G5C59_RS02820	564472..565059	-	588	Protein_547	phage antirepressor N-terminal domain-containing protein	-
G5C59_RS02825	566031..566216	+	186	WP_021118464.1	hypothetical protein	-
G5C59_RS02830	566225..566602	-	378	WP_021118463.1	ECF sigma factor family protein	-
G5C59_RS02835	566599..567003	-	405	WP_005714425.1	RusA family crossover junction endodeoxyribonuclease	-
G5C59_RS02840	567000..567290	-	291	WP_021118462.1	DUF1364 domain-containing protein	-
G5C59_RS02845	567395..567562	+	168	WP_200859958.1	type II toxin-antitoxin system HicA family toxin	Toxin
G5C59_RS02850	567601..568008	+	408	WP_005714429.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
G5C59_RS02855	568042..568491	-	450	WP_164681600.1	recombination protein NinB	-
G5C59_RS02860	568793..569344	-	552	WP_164681601.1	adenine methyltransferase	-
G5C59_RS02865	569345..570700	-	1356	WP_164681602.1	AAA family ATPase	-
G5C59_RS02870	570700..571176	-	477	WP_164681603.1	hypothetical protein	-
G5C59_RS02875	571208..571570	-	363	WP_164681604.1	replication protein	-
G5C59_RS02880	571548..571730	-	183	WP_164681605.1	hypothetical protein	-
G5C59_RS02885	571773..572048	-	276	WP_021110741.1	hypothetical protein	-
G5C59_RS02890	572069..572275	-	207	WP_012621794.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	540481..590366	49885

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (1-55)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 56 a.a.
• Molecular weight: 6272.42 Da
• Isoelectric Point: 11.0319

>T150447 WP_200859958.1 NZ_CP049088:567395-567562 [Glaesserella parasuis]
MIKLLKKNGWELDSIVGSHHHFEKKNQKGAGKVTVPHPRKDLGFLEKKIRKQAGL

Nucleotide

• Length: 168 bp

>T150447 NZ_CP064682:1503543-1503719 [Escherichia coli K-12]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14890.91 Da
• Isoelectric Point: 4.6143

>AT150447 WP_005714429.1 NZ_CP049088:567601-568008 [Glaesserella parasuis]
MLYPLIFEQVSDGYVVAVPDIDGCYSAGDTLEETYSNVKQAIALHLESVVKDGGEIPTPTAIENHKNDPDLDGHNVFFGV
VDVDLSHLMGKAEKINITLPSYLIKRIDDFVAVHKEYKNRSNFLAKIAADKILTA

Nucleotide

• Length: 408 bp

>AT150447 NZ_CP064682:1503765-1504181 [Escherichia coli K-12]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1T0AB61