Detailed information of TA system TA150440

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4713429..4714230
• Replicon: chromosome
• Accession: NZ_CP049085
• Organism: Escherichia coli strain p4A

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D8EBK0
• Locus tag: G6P91_RS23190
• Protein ID: WP_001094436.1
• Coordinates: 4713853..4714230 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MN76
• Locus tag: G6P91_RS23185
• Protein ID: WP_015953067.1
• Coordinates: 4713429..4713806 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6P91_RS23150	4709340..4710020	+	681	WP_001282919.1	WYL domain-containing protein	-
G6P91_RS23155	4710168..4710845	+	678	WP_001097301.1	hypothetical protein	-
G6P91_RS23160	4710851..4711084	+	234	WP_001278287.1	DUF905 family protein	-
G6P91_RS23165	4711174..4711992	+	819	WP_001175163.1	DUF945 domain-containing protein	-
G6P91_RS23170	4712084..4712569	+	486	WP_029700724.1	antirestriction protein	-
G6P91_RS23175	4712584..4713060	+	477	WP_001186756.1	RadC family protein	-
G6P91_RS23180	4713129..4713350	+	222	WP_000692312.1	DUF987 domain-containing protein	-
G6P91_RS23185	4713429..4713806	+	378	WP_015953067.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
G6P91_RS23190	4713853..4714230	+	378	WP_001094436.1	TA system toxin CbtA family protein	Toxin
G6P91_RS23195	4714227..4714715	+	489	WP_000761714.1	hypothetical protein	-
G6P91_RS23200	4714727..4714924	+	198	WP_000839254.1	DUF957 domain-containing protein	-
G6P91_RS23205	4715009..4715704	+	696	Protein_4556	DUF4942 domain-containing protein	-
G6P91_RS23210	4715768..4716523	-	756	WP_000065240.1	IS21-like element ISEc10 family helper ATPase IstB	-
G6P91_RS23215	4716520..4718019	-	1500	WP_029700729.1	IS21-like element ISEc10 family transposase	-
G6P91_RS23220	4718110..4718271	+	162	Protein_4559	DUF4942 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13958.84 Da
• Isoelectric Point: 6.8603

>T150440 WP_001094436.1 NZ_CP049085:4713853-4714230 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T150440 NZ_CP064682:248788-249186 [Escherichia coli K-12]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13766.60 Da
• Isoelectric Point: 6.2021

>AT150440 WP_015953067.1 NZ_CP049085:4713429-4713806 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT150440 NZ_CP064682:248492-248785 [Escherichia coli K-12]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E2L1N0

Antitoxin

• AlphaFold DB: A0A5Z3CIS0