Detailed information of TA system TA150419

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 810226..811060
• Replicon: chromosome
• Accession: NZ_CP049085
• Organism: Escherichia coli strain p4A

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: G6P91_RS03895
• Protein ID: WP_000854690.1
• Coordinates: 810226..810603 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: G6P91_RS03900
• Protein ID: WP_001305076.1
• Coordinates: 810692..811060 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6P91_RS03865	806620..806775	-	156	WP_000729638.1	hypothetical protein	-
G6P91_RS03870	807207..808140	-	934	Protein_760	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
G6P91_RS03875	808133..808528	-	396	WP_000208384.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
G6P91_RS03880	808597..809442	-	846	WP_001529401.1	DUF4942 domain-containing protein	-
G6P91_RS03885	809527..809724	-	198	WP_000839293.1	DUF957 domain-containing protein	-
G6P91_RS03890	809741..810229	-	489	WP_000761699.1	hypothetical protein	-
G6P91_RS03895	810226..810603	-	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
G6P91_RS03900	810692..811060	-	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G6P91_RS03905	811110..811754	-	645	WP_000094916.1	hypothetical protein	-
G6P91_RS03910	811773..811994	-	222	WP_000692329.1	DUF987 domain-containing protein	-
G6P91_RS03915	812057..812533	-	477	WP_001186726.1	RadC family protein	-
G6P91_RS03920	812549..813034	-	486	WP_000849565.1	antirestriction protein	-
G6P91_RS03925	813089..813907	-	819	WP_001234620.1	DUF945 domain-containing protein	-
G6P91_RS03930	814008..814241	-	234	WP_000902034.1	DUF905 family protein	-
G6P91_RS03935	814320..814775	-	456	WP_000581502.1	hypothetical protein	-
G6P91_RS03940	814851..815978	-	1128	Protein_774	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	kpsM / kpsT / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF	788609..813034	24425

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T150419 WP_000854690.1 NZ_CP049085:c810603-810226 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T150419 NZ_CP064681:c1408184-1407990 [Escherichia coli K-12]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT150419 WP_001305076.1 NZ_CP049085:c811060-810692 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT150419 NZ_CP064681:1407813-1407991 [Escherichia coli K-12]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8