Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 289697..290497 Replicon chromosome
Accession NZ_CP049085
Organism Escherichia coli strain p4A

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag G6P91_RS01320 Protein ID WP_000342452.1
Coordinates 289970..290497 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag G6P91_RS01315 Protein ID WP_001277107.1
Coordinates 289697..289963 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6P91_RS01295 285356..286024 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
G6P91_RS01300 286017..287075 + 1059 WP_001042013.1 permease-like cell division protein FtsX -
G6P91_RS01305 287320..288174 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
G6P91_RS01310 288445..289548 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
G6P91_RS01315 289697..289963 + 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
G6P91_RS01320 289970..290497 + 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
G6P91_RS01325 290494..290877 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
G6P91_RS01330 291300..292409 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
G6P91_RS01335 292457..293383 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
G6P91_RS01340 293380..294657 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
G6P91_RS01345 294654..295421 + 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T150416 WP_000342452.1 NZ_CP049085:289970-290497 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T150416 NZ_CP064681:c1264731-1264624 [Escherichia coli K-12]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT150416 WP_001277107.1 NZ_CP049085:289697-289963 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT150416 NZ_CP064681:1264779-1264845 [Escherichia coli K-12]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References