Detailed information of TA system TA150411

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 74426..75051
• Replicon: plasmid p10A_p1
• Accession: NZ_CP049082
• Organism: Escherichia coli strain p10A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6P90_RS23500
• Protein ID: WP_000911313.1
• Coordinates: 74653..75051 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: G6P90_RS23495
• Protein ID: WP_000450520.1
• Coordinates: 74426..74653 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6P90_RS23495	74426..74653	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
G6P90_RS23500	74653..75051	+	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
G6P90_RS23505	75060..77213	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
G6P90_RS23510	77466..78197	-	732	WP_023565183.1	complement resistance protein TraT	-
G6P90_RS23515	78229..78726	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / tet(A) / aph(3')-Ia / sul3 / ant(3'')-Ia / floR / aac(3)-IIa / sitABCD	iutA / iucD / iucC / iucB / iucA	1..143163	143163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T150411 WP_000911313.1 NZ_CP049082:74653-75051 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T150411 NZ_CP064680:c4569655-4569314 [Escherichia coli K-12]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT150411 WP_000450520.1 NZ_CP049082:74426-74653 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT150411 NZ_CP064680:4569650-4569726 [Escherichia coli K-12]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin

• AlphaFold DB: V0VCB2