Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 21113..21756 Replicon plasmid p10A_p1
Accession NZ_CP049082
Organism Escherichia coli strain p10A

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag G6P90_RS23205 Protein ID WP_001044768.1
Coordinates 21340..21756 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag G6P90_RS23200 Protein ID WP_001261287.1
Coordinates 21113..21343 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6P90_RS23180 16273..17361 + 1089 WP_000952217.1 transcriptional repressor PifC -
G6P90_RS23185 17363..19588 + 2226 WP_000698737.1 phage T7 exclusion protein -
G6P90_RS23190 19638..20537 - 900 WP_000963206.1 nucleotide-binding protein -
G6P90_RS23195 20527..20817 - 291 WP_000111771.1 hypothetical protein -
G6P90_RS23200 21113..21343 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G6P90_RS23205 21340..21756 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
G6P90_RS23210 21918..24056 - 2139 WP_000350638.1 AAA family ATPase -
G6P90_RS23215 24581..25843 + 1263 WP_000608644.1 IS1380-like element ISEc9 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-55 / tet(A) / aph(3')-Ia / sul3 / ant(3'')-Ia / floR / aac(3)-IIa / sitABCD iutA / iucD / iucC / iucB / iucA 1..143163 143163


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T150408 WP_001044768.1 NZ_CP049082:21340-21756 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T150408 NZ_CP064680:4342723-4342896 [Escherichia coli K-12]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT150408 WP_001261287.1 NZ_CP049082:21113-21343 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT150408 NZ_CP064680:4342399-4342695 [Escherichia coli K-12]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References