Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4032296..4032872 Replicon chromosome
Accession NZ_CP049081
Organism Escherichia coli strain p10A

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag G6P90_RS19585 Protein ID WP_001297643.1
Coordinates 4032585..4032872 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag G6P90_RS19580 Protein ID WP_000063156.1
Coordinates 4032296..4032598 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6P90_RS19565 4028892..4031042 + 2151 WP_001446922.1 pyruvate/proton symporter BtsT -
G6P90_RS19570 4031092..4031295 + 204 WP_000467859.1 YbdD/YjiX family protein -
G6P90_RS19575 4031306..4032262 + 957 WP_001297640.1 GTPase -
G6P90_RS19580 4032296..4032598 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
G6P90_RS19585 4032585..4032872 - 288 WP_001297643.1 BrnT family toxin Toxin
G6P90_RS19590 4033071..4033768 - 698 Protein_3822 IS1 family transposase -
G6P90_RS19595 4033825..4033920 + 96 Protein_3823 dienelactone hydrolase family protein -
G6P90_RS19600 4034066..4035298 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
G6P90_RS19605 4035339..4036619 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T150407 WP_001297643.1 NZ_CP049081:c4032872-4032585 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T150407 NZ_CP064680:c3693445-3693338 [Escherichia coli K-12]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT150407 WP_000063156.1 NZ_CP049081:c4032598-4032296 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT150407 NZ_CP064680:3693494-3693560 [Escherichia coli K-12]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References