Detailed information of TA system TA150393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2341943..2342581
• Replicon: chromosome
• Accession: NZ_CP049081
• Organism: Escherichia coli strain p10A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: G6P90_RS11395
• Protein ID: WP_001447010.1
• Coordinates: 2341943..2342119 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: G6P90_RS11400
• Protein ID: WP_001270286.1
• Coordinates: 2342165..2342581 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6P90_RS11375	2337562..2338776	-	1215	WP_001326689.1	BenE family transporter YdcO	-
G6P90_RS11380	2338829..2339365	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
G6P90_RS11385	2339438..2341399	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
G6P90_RS11390	2341491..2341721	-	231	WP_000494244.1	YncJ family protein	-
G6P90_RS11395	2341943..2342119	+	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
G6P90_RS11400	2342165..2342581	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
G6P90_RS11405	2342660..2344066	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
G6P90_RS11410	2344311..2345456	+	1146	WP_000047428.1	ABC transporter substrate-binding protein	-
G6P90_RS11415	2345474..2346487	+	1014	WP_000220395.1	ABC transporter ATP-binding protein	-
G6P90_RS11420	2346488..2347429	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T150393 WP_001447010.1 NZ_CP049081:2341943-2342119 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T150393 NZ_CP064680:1503543-1503719 [Escherichia coli K-12]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT150393 WP_001270286.1 NZ_CP049081:2342165-2342581 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT150393 NZ_CP064680:1503765-1504181 [Escherichia coli K-12]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8