Detailed information of TA system TA150387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 176369..176894
• Replicon: plasmid p11A_p2
• Accession: NZ_CP049079
• Organism: Escherichia coli strain p11A

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: G6P89_RS27440
• Protein ID: WP_001159871.1
• Coordinates: 176589..176894 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: H9TJP1
• Locus tag: G6P89_RS27435
• Protein ID: WP_000813630.1
• Coordinates: 176369..176587 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6P89_RS27400	172796..173119	+	324	Protein_195	helix-turn-helix domain-containing protein	-
G6P89_RS27405	173121..173225	+	105	Protein_196	AAA family ATPase	-
G6P89_RS27410	173286..173799	+	514	Protein_197	DUF4113 domain-containing protein	-
G6P89_RS27415	173979..174676	-	698	Protein_198	IS1 family transposase	-
G6P89_RS27420	174732..175091	-	360	Protein_199	AAA family ATPase	-
G6P89_RS27425	175166..175582	-	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	-
G6P89_RS27430	175579..175809	-	231	WP_001261286.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
G6P89_RS27435	176369..176587	+	219	WP_000813630.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
G6P89_RS27440	176589..176894	+	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
G6P89_RS27445	176895..177701	+	807	WP_000016970.1	site-specific integrase	-
G6P89_RS27450	178042..179655	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
G6P89_RS27455	179686..180036	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
G6P89_RS27460	180033..180458	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / mph(A) / sul1 / qacE / aadA5 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15	senB	1..180962	180962
	-	inside	IScluster/Tn	-	-	172796..180458	7662

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T150387 WP_001159871.1 NZ_CP049079:176589-176894 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T150387 NZ_CP064680:c259380-259039 [Escherichia coli K-12]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8354.27 Da
• Isoelectric Point: 4.5203

>AT150387 WP_000813630.1 NZ_CP049079:176369-176587 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT150387 NZ_CP064680:c259718-259401 [Escherichia coli K-12]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A829CCE8

Antitoxin

• AlphaFold DB: A0A829CEF5