Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 175166..175809 | Replicon | plasmid p11A_p2 |
| Accession | NZ_CP049079 | ||
| Organism | Escherichia coli strain p11A | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | V0UN72 |
| Locus tag | G6P89_RS27425 | Protein ID | WP_001034044.1 |
| Coordinates | 175166..175582 (-) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | B1P7N7 |
| Locus tag | G6P89_RS27430 | Protein ID | WP_001261286.1 |
| Coordinates | 175579..175809 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G6P89_RS27395 | 171073..172050 | - | 978 | WP_000361611.1 | RepB family plasmid replication initiator protein | - |
| G6P89_RS27400 | 172796..173119 | + | 324 | Protein_195 | helix-turn-helix domain-containing protein | - |
| G6P89_RS27405 | 173121..173225 | + | 105 | Protein_196 | AAA family ATPase | - |
| G6P89_RS27410 | 173286..173799 | + | 514 | Protein_197 | DUF4113 domain-containing protein | - |
| G6P89_RS27415 | 173979..174676 | - | 698 | Protein_198 | IS1 family transposase | - |
| G6P89_RS27420 | 174732..175091 | - | 360 | Protein_199 | AAA family ATPase | - |
| G6P89_RS27425 | 175166..175582 | - | 417 | WP_001034044.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| G6P89_RS27430 | 175579..175809 | - | 231 | WP_001261286.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| G6P89_RS27435 | 176369..176587 | + | 219 | WP_000813630.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| G6P89_RS27440 | 176589..176894 | + | 306 | WP_001159871.1 | type II toxin-antitoxin system toxin CcdB | - |
| G6P89_RS27445 | 176895..177701 | + | 807 | WP_000016970.1 | site-specific integrase | - |
| G6P89_RS27450 | 178042..179655 | - | 1614 | WP_000080195.1 | IS66-like element ISEc23 family transposase | - |
| G6P89_RS27455 | 179686..180036 | - | 351 | WP_000624722.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| G6P89_RS27460 | 180033..180458 | - | 426 | WP_000422741.1 | IS66 family insertion sequence hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | tet(A) / mph(A) / sul1 / qacE / aadA5 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 | senB | 1..180962 | 180962 | |
| - | inside | IScluster/Tn | - | - | 172796..180458 | 7662 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15232.66 Da Isoelectric Point: 6.8536
>T150386 WP_001034044.1 NZ_CP049079:c175582-175166 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR
Download Length: 417 bp
>T150386 NZ_CP064680:248788-249186 [Escherichia coli K-12]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8747.86 Da Isoelectric Point: 4.3775
>AT150386 WP_001261286.1 NZ_CP049079:c175809-175579 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL
Download Length: 231 bp
>AT150386 NZ_CP064680:248492-248785 [Escherichia coli K-12]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CHW1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CKZ6 |