Detailed information of TA system TA150377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 32216..32763
• Replicon: plasmid p11A_p1
• Accession: NZ_CP049078
• Organism: Escherichia coli strain p11A

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: G6P89_RS26130
• Protein ID: WP_000969996.1
• Coordinates: 32482..32763 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: K4JYR0
• Locus tag: G6P89_RS26125
• Protein ID: WP_000079928.1
• Coordinates: 32216..32485 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6P89_RS26085	27534..27995	+	462	WP_016230918.1	thermonuclease family protein	-
G6P89_RS26090	28041..28250	+	210	WP_001369435.1	hemolysin expression modulator Hha	-
G6P89_RS26095	28288..28515	+	228	Protein_28	hypothetical protein	-
G6P89_RS26100	28532..29229	-	698	Protein_29	IS1 family transposase	-
G6P89_RS26105	29286..29654	+	369	Protein_30	DUF2726 domain-containing protein	-
G6P89_RS26110	29894..30154	+	261	WP_000083819.1	replication regulatory protein RepA	-
G6P89_RS26115	30378..30452	+	75	WP_001365705.1	RepA leader peptide Tap	-
G6P89_RS26120	30445..31302	+	858	WP_042018306.1	incFII family plasmid replication initiator RepA	-
G6P89_RS26125	32216..32485	+	270	WP_000079928.1	hypothetical protein	Antitoxin
G6P89_RS26130	32482..32763	+	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G6P89_RS26135	32809..33657	+	849	WP_016235419.1	3'-5' exonuclease	-
G6P89_RS26140	33774..34256	+	483	WP_001311056.1	hypothetical protein	-
G6P89_RS26145	34616..35803	-	1188	Protein_38	IS91 family transposase	-
G6P89_RS26150	35803..36168	-	366	WP_016235422.1	hypothetical protein	-
G6P89_RS26155	36696..37205	+	510	WP_000115001.1	peptide deformylase	-
G6P89_RS26160	37220..37321	+	102	Protein_41	methionyl-tRNA formyltransferase	-
G6P89_RS26165	37581..37709	+	129	Protein_42	DUF2164 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..70739	70739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T150377 WP_000969996.1 NZ_CP049078:32482-32763 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T150377 NZ_CP064679:c3161903-3161607 [Escherichia coli K-12]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10175.50 Da
• Isoelectric Point: 7.3210

>AT150377 WP_000079928.1 NZ_CP049078:32216-32485 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT150377 NZ_CP064679:c3161605-3161210 [Escherichia coli K-12]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A5W2J4D0