Detailed information of TA system TA150349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1437410..1438049
• Replicon: chromosome
• Accession: NZ_CP049074
• Organism: Metallosphaera tengchongensis strain Ric-A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GWK48_RS07810
• Protein ID: WP_174631133.1
• Coordinates: 1437410..1437835 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GWK48_RS07815
• Protein ID: WP_174631134.1
• Coordinates: 1437819..1438049 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK48_RS07785 (GWK48_07770)	1433148..1433507	-	360	WP_174631126.1	DUF1059 domain-containing protein	-
GWK48_RS07790 (GWK48_07775)	1433600..1433800	+	201	WP_174631128.1	DUF1059 domain-containing protein	-
GWK48_RS07795 (GWK48_07780)	1434524..1434802	+	279	WP_246263779.1	CopG family transcriptional regulator	-
GWK48_RS07800 (GWK48_07785)	1434789..1435193	+	405	WP_174631130.1	type II toxin-antitoxin system VapC family toxin	-
GWK48_RS07805 (GWK48_07790)	1435387..1436715	-	1329	WP_174631131.1	ATP-binding protein	-
GWK48_RS11695	1437015..1437137	+	123	WP_281359966.1	hypothetical protein	-
GWK48_RS07810 (GWK48_07795)	1437410..1437835	-	426	WP_174631133.1	type II toxin-antitoxin system VapC family toxin	Toxin
GWK48_RS07815 (GWK48_07800)	1437819..1438049	-	231	WP_174631134.1	antitoxin	Antitoxin
GWK48_RS07820 (GWK48_07805)	1438335..1438715	-	381	WP_174632681.1	IS200/IS605 family accessory protein TnpB-related protein	-
GWK48_RS07825 (GWK48_07810)	1438774..1438989	-	216	WP_174631136.1	hypothetical protein	-
GWK48_RS07830 (GWK48_07815)	1439234..1440334	+	1101	WP_174631137.1	MFS transporter	-
GWK48_RS07835 (GWK48_07820)	1440331..1440591	+	261	WP_174631139.1	hypothetical protein	-
GWK48_RS07840 (GWK48_07825)	1440837..1442276	+	1440	WP_174631141.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (4-127)

Antitoxin

• Putative antitoxin VapB3-like (1-73)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16092.45 Da
• Isoelectric Point: 5.0365

>T150349 WP_174631133.1 NZ_CP049074:c1437835-1437410 [Metallosphaera tengchongensis]
MRTVVDTSALVALHIPEKLSEYIRDQIEKSEEYHFLDLIYYEFTNVIRKRVARGEISEREAKEILKNGYELISNSTVHEG
KEVIIEAYEMGLKYSITVYDASLVALANKINGKVLTTDLKLLSSLKNTQLYSIFLVGDEVS

Nucleotide

• Length: 426 bp

>T150349 NZ_CP064678:c3034182-3033775 [Escherichia coli K-12]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 9326.58 Da
• Isoelectric Point: 5.2500

>AT150349 WP_174631134.1 NZ_CP049074:c1438049-1437819 [Metallosphaera tengchongensis]
MSEVISIRVSRKLKEDLEELNINYSELVRQYLEQVVRKEKMKRLLQNADTIREELRDRNFIPSYELVRQDRDEDSR

Nucleotide

• Length: 231 bp

>AT150349 NZ_CP064678:c3034429-3034163 [Escherichia coli K-12]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA