Detailed information of TA system TA150345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 325861..326456
• Replicon: chromosome
• Accession: NZ_CP049055
• Organism: Candidatus Kuenenia stuttgartiensis strain CSTR1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: KsCSTR_RS01415
• Protein ID: WP_099325074.1
• Coordinates: 325861..326121 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KsCSTR_RS01420
• Protein ID: WP_099325073.1
• Coordinates: 326118..326456 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KsCSTR_RS01400	322662..323438	-	777	WP_164994387.1	hypothetical protein	-
KsCSTR_RS01405	323983..324684	+	702	WP_164994388.1	carbohydrate ABC transporter permease	-
KsCSTR_RS01410	324681..325760	+	1080	WP_164994389.1	ABC transporter ATP-binding protein	-
KsCSTR_RS01415	325861..326121	+	261	WP_099325074.1	type II toxin-antitoxin system HicA family toxin	Toxin
KsCSTR_RS01420	326118..326456	+	339	WP_099325073.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KsCSTR_RS01425	326606..328162	+	1557	WP_099323647.1	hypothetical protein	-
KsCSTR_RS01430	328681..330318	+	1638	WP_164994390.1	chaperonin GroEL	-
KsCSTR_RS01435	330513..330965	+	453	WP_099325071.1	Hsp20/alpha crystallin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-75)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9890.48 Da
• Isoelectric Point: 10.4214

>T150345 WP_099325074.1 NZ_CP049055:325861-326121 [Candidatus Kuenenia stuttgartiensis]
MSKAEKLLRRFLSKPKDFTYDDLKSLLAGFGYEKAKSGKTTGSGVTFINPETRHIIRLHKPHPGVELKRYQLDYIEEALR
NKGLIQ

Nucleotide

• Length: 261 bp

>T150345 NZ_CP064678:c2083194-2082943 [Escherichia coli K-12]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12582.42 Da
• Isoelectric Point: 7.0617

>AT150345 WP_099325073.1 NZ_CP049055:326118-326456 [Candidatus Kuenenia stuttgartiensis]
MKDVLTYKGFTGTVHFSAEDKVFHGKIEGIDDLVIFEGHSVEELVKAFHNAADDYAALCREMGKVPIKSYRGSFNVRIPP
EMHRKAAERAIQKGLSLNQLVQTAIEKELVHN

Nucleotide

• Length: 339 bp

>AT150345 NZ_CP064678:c2082946-2082692 [Escherichia coli K-12]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA