Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 75180..75823 Replicon plasmid p233-142
Accession NZ_CP049048
Organism Enterobacter hormaechei strain Y233

Toxin (Protein)


Gene name vagD Uniprot ID A0A5P6KDG5
Locus tag G5B34_RS24670 Protein ID WP_021312768.1
Coordinates 75180..75596 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A5P6KGW8
Locus tag G5B34_RS24675 Protein ID WP_021312767.1
Coordinates 75593..75823 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5B34_RS24645 71469..72353 + 885 WP_000155092.1 Mph(E) family macrolide 2'-phosphotransferase -
G5B34_RS24650 72712..73254 - 543 WP_004896925.1 plasmid replication DNA-binding protein -
G5B34_RS24655 73321..74073 - 753 Protein_83 replication initiation protein RepM -
G5B34_RS24660 74139..74843 + 705 WP_016162063.1 IS6-like element IS26 family transposase -
G5B34_RS24665 74849..75082 + 234 WP_089617563.1 helix-turn-helix domain-containing protein -
G5B34_RS24670 75180..75596 - 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin Toxin
G5B34_RS24675 75593..75823 - 231 WP_021312767.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G5B34_RS24680 75877..76281 + 405 Protein_88 hypothetical protein -
G5B34_RS24685 76432..76743 + 312 WP_042948507.1 hypothetical protein -
G5B34_RS24690 76863..77423 + 561 WP_042948505.1 hypothetical protein -
G5B34_RS24695 77602..78378 + 777 WP_016338374.1 site-specific integrase -
G5B34_RS24700 78821..79693 - 873 WP_042948503.1 RepB family plasmid replication initiator protein -
G5B34_RS24705 80227..80463 - 237 WP_016338376.1 ParG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / ant(3'')-Ia / qacE / sul1 / armA / msr(E) / mph(E) mrkA / mrkB / mrkC / mrkF 1..142557 142557


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15175.60 Da        Isoelectric Point: 7.2452

>T150344 WP_021312768.1 NZ_CP049048:c75596-75180 [Enterobacter hormaechei]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPDLVLEDWVR

Download         Length: 417 bp

>T150344 NZ_CP064678:c2082946-2082692 [Escherichia coli K-12]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8722.81 Da        Isoelectric Point: 4.4692

>AT150344 WP_021312767.1 NZ_CP049048:c75823-75593 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT150344 NZ_CP064678:c2083194-2082943 [Escherichia coli K-12]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5P6KDG5

Antitoxin

Source ID Structure
AlphaFold DB A0A5P6KGW8

References