Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 301853..302379 Replicon plasmid pIHI2-233
Accession NZ_CP049047
Organism Enterobacter hormaechei strain Y233

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag G5B34_RS24125 Protein ID WP_000323025.1
Coordinates 302092..302379 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag G5B34_RS24120 Protein ID WP_000534858.1
Coordinates 301853..302092 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5B34_RS24105 298181..299419 + 1239 WP_000219087.1 IS110 family transposase -
G5B34_RS24110 299895..300467 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
G5B34_RS24115 300667..301590 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
G5B34_RS24120 301853..302092 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
G5B34_RS24125 302092..302379 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
G5B34_RS24130 302451..302609 + 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
G5B34_RS24135 303218..303538 - 321 WP_000332796.1 hypothetical protein -
G5B34_RS24140 303820..304023 - 204 WP_001015183.1 hypothetical protein -
G5B34_RS24145 304070..304420 - 351 WP_000743059.1 hypothetical protein -
G5B34_RS24150 304480..305079 - 600 Protein_337 hypothetical protein -
G5B34_RS24155 305178..306122 - 945 WP_000778029.1 DUF5417 domain-containing protein -
G5B34_RS24160 306633..306809 + 177 WP_001371930.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / mcr-9 / aph(6)-Id / aph(3'')-Ib / dfrA19 / blaTEM-1B / aac(6')-IIc / ere(A) / qacE / sul1 / qnrB4 / blaDHA-1 / blaSHV-12 / catA2 / tet(D) - 1..322325 322325


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T150342 WP_000323025.1 NZ_CP049047:302092-302379 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T150342 NZ_CP064678:c1917464-1917361 [Escherichia coli K-12]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT150342 WP_000534858.1 NZ_CP049047:301853-302092 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT150342 NZ_CP064678:1917323-1917594 [Escherichia coli K-12]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References