Detailed information of TA system TA150320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5242083..5242599
• Replicon: chromosome
• Accession: NZ_CP049044
• Organism: Pseudomonas psychrophila strain KM02

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A8I1FXC2
• Locus tag: G5J76_RS23830
• Protein ID: WP_019827368.1
• Coordinates: 5242312..5242599 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8I1FYB3
• Locus tag: G5J76_RS23825
• Protein ID: WP_019827369.1
• Coordinates: 5242083..5242322 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5J76_RS23805	5237987..5239081	-	1095	WP_053070228.1	ABC transporter substrate-binding protein	-
G5J76_RS23810	5239118..5240029	-	912	WP_019827371.1	TauD/TfdA family dioxygenase	-
G5J76_RS23815	5240232..5240921	-	690	WP_048352522.1	NYN domain-containing protein	-
G5J76_RS23820	5241244..5241591	-	348	WP_048352521.1	hypothetical protein	-
G5J76_RS23825	5242083..5242322	+	240	WP_019827369.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
G5J76_RS23830	5242312..5242599	+	288	WP_019827368.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G5J76_RS23835	5242722..5243933	+	1212	WP_048352520.1	ABC transporter substrate-binding protein	-
G5J76_RS23840	5243948..5244523	+	576	WP_019827366.1	ANTAR domain-containing protein	-
G5J76_RS23845	5244831..5246042	+	1212	WP_048352519.1	NarK/NasA family nitrate transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11347.25 Da
• Isoelectric Point: 10.3151

>T150320 WP_019827368.1 NZ_CP049044:5242312-5242599 [Pseudomonas psychrophila]
MTFDLEFDQRALKEWHKLADTVRLQFKSKLSEVLLNPRVEANRLRKLPDCYKIKLRSAGYRLIYQVIDQEVVVFVVAVDK
REHQAAYRKAQDRLK

Nucleotide

• Length: 288 bp

>T150320 NZ_CP064674:879914-880312 [Salmonella sp. SJTUF14076]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9063.45 Da
• Isoelectric Point: 4.8279

>AT150320 WP_019827369.1 NZ_CP049044:5242083-5242322 [Pseudomonas psychrophila]
MASINIRIDDELKARAYKELERLGVTPSELVRQALQYVAERGQLPFRPMLVTEEDEALINTVRERLAAPQRVKVTLDDL

Nucleotide

• Length: 240 bp

>AT150320 NZ_CP064674:879687-879914 [Salmonella sp. SJTUF14076]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGATGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA