Detailed information of TA system TA150316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 24673..25316
• Replicon: plasmid pEclE3-2
• Accession: NZ_CP049023
• Organism: Enterobacter hormaechei strain Eho-E3

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: GVI75_RS26670
• Protein ID: WP_000754566.1
• Coordinates: 24673..25089 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: GVI75_RS26675
• Protein ID: WP_001261276.1
• Coordinates: 25086..25316 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI75_RS26645	21023..21727	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
GVI75_RS26650	21790..21882	+	93	Protein_23	IS5/IS1182 family transposase	-
GVI75_RS26655	21890..22198	-	309	Protein_24	DDE-type integrase/transposase/recombinase	-
GVI75_RS26660	22215..23291	-	1077	WP_000227969.1	IS110 family transposase	-
GVI75_RS26665	23573..24429	-	857	Protein_26	IS3-like element ISEc15 family transposase	-
GVI75_RS26670	24673..25089	-	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
GVI75_RS26675	25086..25316	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GVI75_RS26680	25905..26198	+	294	WP_020956883.1	hypothetical protein	-
GVI75_RS26685	26262..26948	+	687	WP_020956882.1	hypothetical protein	-
GVI75_RS26690	26960..27736	+	777	WP_020956881.1	site-specific integrase	-
GVI75_RS26695	27794..28051	-	258	WP_000764642.1	hypothetical protein	-
GVI75_RS26700	28180..28284	-	105	WP_032409716.1	hypothetical protein	-
GVI75_RS26705	28814..29680	+	867	WP_004118283.1	replication initiation protein	-
GVI75_RS26710	29857..30126	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / blaVIM-1 / ant(3'')-Ia / qacE / sul1 / mph(A)	galE	1..83090	83090

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T150316 WP_000754566.1 NZ_CP049023:c25089-24673 [Enterobacter hormaechei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T150316 NZ_CP064672:c4592963-4592589 [Salmonella sp. SJTUF14146]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAGAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAGCGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
TAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT150316 WP_001261276.1 NZ_CP049023:c25316-25086 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT150316 NZ_CP064672:c4593205-4592963 [Salmonella sp. SJTUF14146]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023