Detailed information of TA system TA150315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 117004..118627
• Replicon: plasmid pEclE3-1
• Accession: NZ_CP049022
• Organism: Enterobacter hormaechei strain Eho-E3

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C1IUI3
• Locus tag: GVI75_RS25405
• Protein ID: WP_000088044.1
• Coordinates: 117302..118627 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6M3PBR7
• Locus tag: GVI75_RS25400
• Protein ID: WP_012477377.1
• Coordinates: 117004..117297 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI75_RS25380	112803..113195	+	393	WP_000341066.1	hypothetical protein	-
GVI75_RS25385	113854..114510	+	657	WP_000462754.1	trypsin-like serine protease	-
GVI75_RS25390	114713..115210	+	498	WP_000340139.1	membrane protein	-
GVI75_RS25395	115215..116603	+	1389	WP_000083579.1	hypothetical protein	-
GVI75_RS25400	117004..117297	+	294	WP_012477377.1	helix-turn-helix transcriptional regulator	Antitoxin
GVI75_RS25405	117302..118627	+	1326	WP_000088044.1	type II toxin-antitoxin system HipA family toxin	Toxin
GVI75_RS25410	118688..118894	+	207	WP_000134171.1	hypothetical protein	-
GVI75_RS25415	118995..119405	+	411	WP_000985909.1	hypothetical protein	-
GVI75_RS25420	119418..120233	+	816	WP_001641519.1	HNH endonuclease	-
GVI75_RS25425	120486..120911	+	426	WP_000278471.1	hypothetical protein	-
GVI75_RS25430	121460..121768	+	309	WP_001224686.1	hypothetical protein	-
GVI75_RS25435	121784..122641	+	858	WP_000784387.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaACC-1 / sul1 / aph(6)-Id / aph(3'')-Ib / mcr-9 / qacE	-	1..331872	331872

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50333.32 Da
• Isoelectric Point: 6.5044

>T150315 WP_000088044.1 NZ_CP049022:117302-118627 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T150315 NZ_CP064672:c4172392-4172108 [Salmonella sp. SJTUF14146]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10860.30 Da
• Isoelectric Point: 10.3134

>AT150315 WP_012477377.1 NZ_CP049022:117004-117297 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT150315 NZ_CP064672:c4172624-4172382 [Salmonella sp. SJTUF14146]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTACGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A2S1XXW1

Antitoxin

• AlphaFold DB: A0A6M3PBR7