Detailed information of TA system TA150314

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 5029316..5029932
• Replicon: chromosome
• Accession: NZ_CP049021
• Organism: Enterobacter hormaechei strain Eho-E3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GVI75_RS24440
• Protein ID: WP_015569913.1
• Coordinates: 5029316..5029687 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: GVI75_RS24445
• Protein ID: WP_015569912.1
• Coordinates: 5029690..5029932 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI75_RS24425	5026816..5027718	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
GVI75_RS24430	5027715..5028350	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
GVI75_RS24435	5028347..5029276	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
GVI75_RS24440	5029316..5029687	-	372	WP_015569913.1	PIN domain-containing protein	Toxin
GVI75_RS24445	5029690..5029932	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
GVI75_RS24450	5030131..5031051	+	921	WP_015569911.1	alpha/beta hydrolase	-
GVI75_RS24455	5031060..5032001	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
GVI75_RS24460	5032046..5032483	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GVI75_RS24465	5032480..5033361	-	882	WP_003861949.1	virulence factor BrkB family protein	-
GVI75_RS24470	5033355..5033954	-	600	WP_003861947.1	glucose-1-phosphatase	-
GVI75_RS24475	5034073..5034873	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13767.90 Da
• Isoelectric Point: 6.4882

>T150314 WP_015569913.1 NZ_CP049021:c5029687-5029316 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVSARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T150314 NZ_CP064672:3674613-3674840 [Salmonella sp. SJTUF14146]
ATGACCACCTATTACTCGCGCATCCCCAGCCTGCACCTTAAGGGCGACTGGCTGGCAGAAGCGGGATTTGATACCGGGGC
GAGCGTGACCGTGAAGATCTCCGAAGGGTGTTTGACTCTGATTGTGGAGACAGACGAAGTGAGGGATCTACGGAGGGAGC
TTTATCAGGTGAAAAAGTCGATGAAGCACATTAAGGCGGGGGTATATGATGTGGTGAACGGGAATTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT150314 WP_015569912.1 NZ_CP049021:c5029932-5029690 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT150314 NZ_CP064672:c3674830-3674605 [Salmonella sp. SJTUF14146]
TTCACCACATCATATACCCCCGCCTTAATGTGCTTCATCGACTTTTTCACCTGATAAAGCTCCCTCCGTAGATCCCTCAC
TTCGTCTGTCTCCACAATCAGAGTCAAACACCCTTCGGAGATCTTCACGGTCACGCTCGCCCCGGTATCAAATCCCGCTT
CTGCCAGCCAGTCGCCCTTAAGGTGCAGGCTGGGGATGCGCGAGTAATAGGTGGTCATGTGGGTTT

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3