Detailed information of TA system TA150247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 185007..185645
• Replicon: chromosome
• Accession: NZ_CP048934
• Organism: Escherichia coli strain 190693

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: G5C00_RS00865
• Protein ID: WP_000813795.1
• Coordinates: 185007..185183 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: G5C00_RS00870
• Protein ID: WP_076797675.1
• Coordinates: 185229..185645 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5C00_RS00840	180094..180594	+	501	Protein_166	DUF3313 domain-containing protein	-
G5C00_RS00845	180629..181840	-	1212	WP_071591517.1	BenE family transporter YdcO	-
G5C00_RS00850	181893..182429	+	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
G5C00_RS00855	182502..184463	+	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
G5C00_RS00860	184555..184785	-	231	WP_023910283.1	YncJ family protein	-
G5C00_RS00865	185007..185183	+	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
G5C00_RS00870	185229..185645	+	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
G5C00_RS00875	185724..187130	+	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
G5C00_RS00880	187375..188520	+	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
G5C00_RS00885	188538..189551	+	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
G5C00_RS00890	189552..190493	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T150247 WP_000813795.1 NZ_CP048934:185007-185183 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T150247 NZ_CP064424:513113-513454 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT150247 WP_076797675.1 NZ_CP048934:185229-185645 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT150247 NZ_CP064424:512883-513113 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1