Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5440894..5441519 Replicon chromosome
Accession NZ_CP048933
Organism Klebsiella pneumoniae strain KP18-2079

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag G5B45_RS27575 Protein ID WP_002882817.1
Coordinates 5440894..5441277 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag G5B45_RS27580 Protein ID WP_004150355.1
Coordinates 5441277..5441519 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5B45_RS27560 5438260..5439162 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
G5B45_RS27565 5439159..5439794 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
G5B45_RS27570 5439791..5440720 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
G5B45_RS27575 5440894..5441277 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
G5B45_RS27580 5441277..5441519 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
G5B45_RS27585 5441724..5442641 + 918 WP_002882812.1 alpha/beta hydrolase -
G5B45_RS27590 5442655..5443596 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
G5B45_RS27595 5443641..5444078 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
G5B45_RS27600 5444075..5444935 - 861 WP_002882807.1 virulence factor BrkB family protein -
G5B45_RS27605 5444929..5445528 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T150246 WP_002882817.1 NZ_CP048933:c5441277-5440894 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T150246 NZ_CP064424:217108-217374 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT150246 WP_004150355.1 NZ_CP048933:c5441519-5441277 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT150246 NZ_CP064424:216845-217108 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References