Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1108770..1109424 Replicon chromosome
Accession NZ_CP048920
Organism Escherichia coli strain SCU-101

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag G4235_RS05440 Protein ID WP_000244781.1
Coordinates 1109017..1109424 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G4235_RS05435 Protein ID WP_000354046.1
Coordinates 1108770..1109036 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4235_RS05415 1104858..1106291 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
G4235_RS05420 1106336..1106647 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
G4235_RS05425 1106811..1107470 + 660 WP_000250274.1 hemolysin III family protein -
G4235_RS05430 1107547..1108527 - 981 WP_000886079.1 tRNA-modifying protein YgfZ -
G4235_RS05435 1108770..1109036 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G4235_RS05440 1109017..1109424 + 408 WP_000244781.1 protein YgfX Toxin
G4235_RS05445 1109464..1109985 - 522 WP_001055874.1 flavodoxin FldB -
G4235_RS05450 1110097..1110993 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G4235_RS05455 1111018..1111728 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G4235_RS05460 1111734..1113467 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T150200 WP_000244781.1 NZ_CP048920:1109017-1109424 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T150200 NZ_CP064405:c756682-756374 [Mycobacterium tuberculosis variant bovis BCG]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT150200 WP_000354046.1 NZ_CP048920:1108770-1109036 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT150200 NZ_CP064405:c756914-756669 [Mycobacterium tuberculosis variant bovis BCG]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References