Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4235171..4235668 Replicon chromosome
Accession NZ_CP048915
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G5S48_RS20410 Protein ID WP_001173343.1
Coordinates 4235171..4235344 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag G5S48_RS20415 Protein ID WP_001425728.1
Coordinates 4235372..4235668 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S48_RS20400 (4231841) 4231841..4233298 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
G5S48_RS20405 (4233535) 4233535..4235052 + 1518 WP_001295074.1 lysine--tRNA ligase -
G5S48_RS20410 (4235171) 4235171..4235344 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G5S48_RS20415 (4235372) 4235372..4235668 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G5S48_RS20420 (4235895) 4235895..4236167 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
G5S48_RS20425 (4236179) 4236179..4236409 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
G5S48_RS20430 (4236590) 4236590..4238221 + 1632 WP_001216487.1 sensor histidine kinase -
G5S48_RS20435 (4238218) 4238218..4238937 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T150190 WP_001173343.1 NZ_CP048915:c4235344-4235171 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T150190 NZ_CP064405:365346-365771 [Mycobacterium tuberculosis variant bovis BCG]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT150190 WP_001425728.1 NZ_CP048915:c4235668-4235372 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT150190 NZ_CP064405:365128-365349 [Mycobacterium tuberculosis variant bovis BCG]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References