Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3488458..3489076 Replicon chromosome
Accession NZ_CP048915
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag G5S48_RS16815 Protein ID WP_001291435.1
Coordinates 3488858..3489076 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag G5S48_RS16810 Protein ID WP_000344800.1
Coordinates 3488458..3488832 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S48_RS16800 (3483547) 3483547..3484740 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
G5S48_RS16805 (3484763) 3484763..3487912 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
G5S48_RS16810 (3488458) 3488458..3488832 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
G5S48_RS16815 (3488858) 3488858..3489076 + 219 WP_001291435.1 HHA domain-containing protein Toxin
G5S48_RS16820 (3489248) 3489248..3489799 + 552 WP_000102568.1 maltose O-acetyltransferase -
G5S48_RS16825 (3489915) 3489915..3490385 + 471 WP_000136192.1 YlaC family protein -
G5S48_RS16830 (3490549) 3490549..3492099 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
G5S48_RS16835 (3492141) 3492141..3492494 - 354 WP_000878140.1 DUF1428 domain-containing protein -
G5S48_RS16845 (3492873) 3492873..3493184 + 312 WP_000409911.1 MGMT family protein -
G5S48_RS16850 (3493215) 3493215..3493787 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T150189 WP_001291435.1 NZ_CP048915:3488858-3489076 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T150189 NZ_CP064405:364778-365080 [Mycobacterium tuberculosis variant bovis BCG]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT150189 WP_000344800.1 NZ_CP048915:3488458-3488832 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT150189 NZ_CP064405:365128-365349 [Mycobacterium tuberculosis variant bovis BCG]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References