Detailed information of TA system TA150188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 2801496..2803216
• Replicon: chromosome
• Accession: NZ_CP048915
• Organism: Escherichia coli strain E2

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A7U9J2A7
• Locus tag: G5S48_RS13565
• Protein ID: WP_000312833.1
• Coordinates: 2801496..2802152 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: B7UP19
• Locus tag: G5S48_RS13570
• Protein ID: WP_000634203.1
• Coordinates: 2802149..2803216 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S48_RS13550 (2797411)	2797411..2797650	+	240	WP_024224214.1	hypothetical protein	-
G5S48_RS13555 (2797690)	2797690..2800128	+	2439	WP_032180025.1	type I restriction-modification system subunit M	-
G5S48_RS13560 (2800128)	2800128..2801483	+	1356	WP_223349791.1	restriction endonuclease subunit S	-
G5S48_RS13565 (2801496)	2801496..2802152	+	657	WP_000312833.1	toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT	Toxin
G5S48_RS13570 (2802149)	2802149..2803216	+	1068	WP_000634203.1	toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG	Antitoxin
G5S48_RS13575 (2803235)	2803235..2806330	+	3096	WP_000108736.1	HsdR family type I site-specific deoxyribonuclease	-
G5S48_RS13580 (2806330)	2806330..2807046	+	717	WP_001122107.1	SprT family zinc-dependent metalloprotease	-
G5S48_RS13585 (2807391)	2807391..2808005	+	615	Protein_2667	IS3-like element IS600 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	blaCMY-2	-	2794058..2824337	30279

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25233.56 Da
• Isoelectric Point: 6.2145

>T150188 WP_000312833.1 NZ_CP048915:2801496-2802152 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Nucleotide

• Length: 657 bp

>T150188 NZ_CP064405:364778-365080 [Mycobacterium tuberculosis variant bovis BCG]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40672.70 Da
• Isoelectric Point: 5.6927

>AT150188 WP_000634203.1 NZ_CP048915:2802149-2803216 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT150188 NZ_CP064405:364554-364781 [Mycobacterium tuberculosis variant bovis BCG]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9J2A7

Antitoxin

• AlphaFold DB: A0A7U9J2B9