Detailed information of TA system TA150176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1317076..1317701
• Replicon: chromosome
• Accession: NZ_CP048915
• Organism: Escherichia coli strain E2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G5S48_RS06390
• Protein ID: WP_000911330.1
• Coordinates: 1317303..1317701 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: G5S48_RS06385
• Protein ID: WP_000450524.1
• Coordinates: 1317076..1317303 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S48_RS06360 (1312879)	1312879..1313349	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
G5S48_RS06365 (1313349)	1313349..1313921	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
G5S48_RS06370 (1314067)	1314067..1314945	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
G5S48_RS06375 (1314962)	1314962..1315996	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
G5S48_RS06380 (1316209)	1316209..1316922	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
G5S48_RS06385 (1317076)	1317076..1317303	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
G5S48_RS06390 (1317303)	1317303..1317701	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
G5S48_RS06395 (1317848)	1317848..1318711	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
G5S48_RS06400 (1318726)	1318726..1320741	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
G5S48_RS06405 (1320815)	1320815..1321156	+	342	Protein_1253	esterase	-
G5S48_RS06410 (1321238)	1321238..1321984	-	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T150176 WP_000911330.1 NZ_CP048915:1317303-1317701 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T150176 NZ_CP064403:c142914-142633 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT150176 WP_000450524.1 NZ_CP048915:1317076-1317303 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT150176 NZ_CP064403:c143138-142911 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33