Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 313646..314446 Replicon chromosome
Accession NZ_CP048915
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag G5S48_RS01450 Protein ID WP_000342449.1
Coordinates 313919..314446 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag G5S48_RS01445 Protein ID WP_001277108.1
Coordinates 313646..313912 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S48_RS01425 (309303) 309303..309971 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
G5S48_RS01430 (309964) 309964..311022 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
G5S48_RS01435 (311267) 311267..312121 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
G5S48_RS01440 (312393) 312393..313496 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
G5S48_RS01445 (313646) 313646..313912 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
G5S48_RS01450 (313919) 313919..314446 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
G5S48_RS01455 (314443) 314443..314826 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
G5S48_RS01460 (315250) 315250..316359 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
G5S48_RS01465 (316407) 316407..317333 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
G5S48_RS01470 (317330) 317330..318607 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
G5S48_RS01475 (318604) 318604..319371 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T150170 WP_000342449.1 NZ_CP048915:313919-314446 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T150170 NZ_CP064401:c4650879-4650124 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGCTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGTGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT150170 WP_001277108.1 NZ_CP048915:313646-313912 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT150170 NZ_CP064401:c4651382-4651014 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCCGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGAGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References